Western Blot (WB)
(Western Blot analysis of LSD1 in P815 cell lysate with antibody at (A) 1ug/ml and (B) 2ug/ml.)
Rabbit LSD1 Polyclonal Antibody
LSD1, NT (Lysine-Specific Histone Demethylase, Amine Oxidase Flavin-containing Domain Protein 2, AOF2)
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
LSD1; Polyclonal Antibody; NT (Lysine-Specific Histone Demethylase; Amine Oxidase Flavin-containing Domain Protein 2; AOF2); Anti -LSD1; anti-LSD1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human LSD1
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Applicable Applications for anti-LSD1 antibody
Western Blot (WB)
Application Notes
Dilution: Western Blot: 1-2ug'ml
Immunogen
Synthetic peptide LSD1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human LSD1 (Genbank accession No. NP_001009999).
Positive Control
P815 cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis of LSD1 in P815 cell lysate with antibody at (A) 1ug/ml and (B) 2ug/ml.)
Western Blot (WB)
(Western Blot analysis of LSD1 in P815 cell lysate with antibody at (A) 1ug/ml and (B) 2ug/ml.)
Related Product Information for anti-LSD1 antibody
Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine- specific demethylation of histone proteins via an FAD- dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various
proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3- K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5)
acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Applications:
Suitable for use in Western Blot. Other applications not tested.
proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3- K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5)
acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Product Categories/Family for anti-LSD1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The LSD1 (Catalog #AAA610074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LSD1, NT (Lysine-Specific Histone Demethylase, Amine Oxidase Flavin-containing Domain Protein 2, AOF2) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LSD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Dilution: Western Blot: 1-2ug'ml. Researchers should empirically determine the suitability of the LSD1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LSD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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