Rabbit cdk9, p43 Polyclonal Antibody | anti-Cdk9 antibody
cdk9, p43 (PITALRE, cdc Kinase Family, Cyclin-dependent Kinase 9)
Gene Names
Cdk9; cdk9; CDK9; CG5179; DmelCG5179; P-TEF; P-TEFb; PTefb
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
SerumSerum
Synonyms
cdk9; p43; Polyclonal Antibody; p43 (PITALRE; cdc Kinase Family; Cyclin-dependent Kinase 9); Anti -cdk9; anti-Cdk9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human cdk9 (PITALRE) protein at 43kD. No reaction was observed against other related cyclin dependent kinases. Species crossreactivity: rat and mouse. The murine cDNA is shown to be 98% identical with human.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Applicable Applications for anti-Cdk9 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: ELISA: 1:10,000-1:50,000
Western Blot: 1:500-1:3000
Immunohistochemistry (Paraffin): 1:200-1:1000
Dilution: ELISA: 1:10,000-1:50,000
Western Blot: 1:500-1:3000
Immunohistochemistry (Paraffin): 1:200-1:1000
Immunogen
Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE)
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Cdk9 antibody
The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1. The cyclin T1/cdk9 (PITALRE) complex phosphorylates the carboxy terminal domain of RNA Polymerase II in vitro and associate with HIV-1 generated Tat in HIV-1 infected T-cells. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9 (PITALRE) is one of the HIV-1 required host cellular cofactors generated during T-cell activation. Cyclin T1/cdk9 (PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. The cdk9 (PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev.
Product Categories/Family for anti-Cdk9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Cyclin-dependent kinase 9
NCBI Official Synonym Full Names
Cyclin-dependent kinase 9
NCBI Official Symbol
Cdk9
NCBI Official Synonym Symbols
cdk9; CDK9; CG5179; DmelCG5179; P-TEF; P-TEFb; PTefb
NCBI Protein Information
CG5179 gene product from transcript CG5179-RA; Cyclin-dependent kinase 9; CG5179-PA; Cdk9-PA; positive transcription elongation factor b, small subunit; Cyclin-dependent kinase9 2.7; p43 PITALRE
Protein Family
Research Articles on Cdk9
Similar Products
Product Notes
The Cdk9 (Catalog #AAA615997) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The cdk9, p43 (PITALRE, cdc Kinase Family, Cyclin-dependent Kinase 9) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's cdk9, p43 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Dilution: ELISA: 1:10,000-1:50,000 Western Blot: 1:500-1:3000 Immunohistochemistry (Paraffin): 1:200-1:1000. Researchers should empirically determine the suitability of the Cdk9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cdk9, p43, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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