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Western Blot (WB) (WB Suggested Anti-NEK9 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: THP-1 cell lysate)

Rabbit NEK9 Polyclonal Antibody | anti-NEK9 antibody

NEK9 antibody - middle region

Gene Names
NEK9; NC; APUG; NERCC; LCCS10; NERCC1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
Western Blot
Purity
Affinity Purified
Synonyms
NEK9; Polyclonal Antibody; NEK9 antibody - middle region; anti-NEK9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: GGGGGGEEEDSQQESETPDPSGGFRGTMEADRGMEGLISPTEAMGNSNGA
Sequence Length
979
Applicable Applications for anti-NEK9 antibody
Western Blot (WB)
Homology
Cow: 86%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human NEK9
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(WB Suggested Anti-NEK9 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: THP-1 cell lysate)

Western Blot (WB) (WB Suggested Anti-NEK9 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: THP-1 cell lysate)
Related Product Information for anti-NEK9 antibody
This is a rabbit polyclonal antibody against NEK9. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: NEK9 is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. NEK9 phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. NEK9 phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
Product Categories/Family for anti-NEK9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
107kDa
NCBI Official Full Name
serine/threonine-protein kinase Nek9 isoform 2
NCBI Official Synonym Full Names
NIMA related kinase 9
NCBI Official Symbol
NEK9
NCBI Official Synonym Symbols
NC; APUG; NERCC; LCCS10; NERCC1
NCBI Protein Information
serine/threonine-protein kinase Nek9
UniProt Protein Name
Serine/threonine-protein kinase Nek9
UniProt Gene Name
NEK9
UniProt Synonym Gene Names
KIAA1995; NEK8; NERCC; NimA-related protein kinase 9; Nek8
UniProt Entry Name
NEK9_HUMAN

NCBI Description

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression. [provided by RefSeq, Jul 2016]

Uniprot Description

NEK9: a protein kinase related to the NIMA (never in mitosis, gene A) serine/threonine kinase of Aspergillus nidulans. Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation.

Protein type: Protein kinase, Other; Autophagy; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; NEK family

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein kinase binding; ATP binding

Biological Process: mitosis; cell division; mitotic nuclear envelope disassembly; mitotic cell cycle; protein amino acid phosphorylation

Research Articles on NEK9

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Product Notes

The NEK9 nek9 (Catalog #AAA3209269) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEK9 antibody - middle region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NEK9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the NEK9 nek9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: GGGGGGEEED SQQESETPDP SGGFRGTMEA DRGMEGLISP TEAMGNSNGA. It is sometimes possible for the material contained within the vial of "NEK9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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