Rabbit anti-Human, Mouse Cdk9 Polyclonal Antibody | anti-CDK9 antibody

Cdk9, CT (Cdk9, Cyclin-dependent kinase 9, Cell division protein kinase 9)

Cat. Num. AAA643007

• Gene Names: CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Cdk9; Polyclonal Antibody; CT (Cdk9; Cyclin-dependent kinase 9; Cell division protein kinase 9); Anti -Cdk9; anti-CDK9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CDK9 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: Mouse Cdk9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of Mouse Cdk9.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Cdk9 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Cdk9 antibody detected the Cdk9 protein (arrow).)

Western Blot (WB)

(Mouse Cdk9 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Cdk9 antibody detected the Cdk9 protein (arrow).)

Related Product Information for anti-CDK9 antibody

Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro (By similarity).

Product Categories/Family for anti-CDK9 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4502747
• NCBI GeneID: 1025
• NCBI Accession #: NP_001252.1
• NCBI GenBank Nucleotide #: NM_001261.3
• UniProt Accession #: P50750; Q5JU24; Q5JU25; Q5U006; Q96TF1
• Molecular Weight: 42,778 Da
• NCBI Official Full Name: cyclin-dependent kinase 9
• NCBI Official Synonym Full Names: cyclin-dependent kinase 9
• NCBI Official Symbol: CDK9
• NCBI Official Synonym Symbols: TAK; C-2k; CTK1; CDC2L4; PITALRE
• NCBI Protein Information: cyclin-dependent kinase 9; CDC2-related kinase; cell division protein kinase 9; serine/threonine protein kinase PITALRE; serine/threonine-protein kinase PITALRE; cell division cycle 2-like protein kinase 4; tat-associated kinase complex catalytic subunit
• UniProt Protein Name: Cyclin-dependent kinase 9
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK9
• UniProt Synonym Gene Names: CDC2L4; TAK
• UniProt Entry Name: CDK9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol.

Protein type: Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Nuclear receptor co-regulator; EC 2.7.11.22; Kinase, protein; Cell cycle regulation; EC 2.7.11.23; CMGC group; CDK family; CDK9 subfamily; CDK/CDK9 subfamily

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: RNA polymerase subunit kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; chromatin binding; ATP binding; protein kinase activity

Biological Process: regulation of histone modification; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of histone phosphorylation; transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; regulation of mitotic cell cycle; DNA repair; protein amino acid phosphorylation; regulation of DNA repair; cell proliferation; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; replication fork arrest; gene expression