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Western Blot (WB) (Mouse Cdk9 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Cdk9 antibody detected the Cdk9 protein (arrow).)

Rabbit anti-Human, Mouse Cdk9 Polyclonal Antibody | anti-Cdk9 antibody

Cdk9, CT (Cdk9, Cyclin-dependent kinase 9, Cell division protein kinase 9) (AP)

Gene Names
Cdk9; PITALRE
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Cdk9; Polyclonal Antibody; CT (Cdk9; Cyclin-dependent kinase 9; Cell division protein kinase 9) (AP); Cell division protein kinase 9; anti-Cdk9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Cdk9. Species crossreactivity: mouse
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-Cdk9 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to a portion within aa251-278 from the C-terminal region of Mouse Cdk9 (NP_570930), conjugated to KLH. Species sequence homology: rat, bovine
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Western Blot (WB)

(Mouse Cdk9 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Cdk9 antibody detected the Cdk9 protein (arrow).)

Western Blot (WB) (Mouse Cdk9 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Cdk9 antibody detected the Cdk9 protein (arrow).)

Western Blot (WB)

(Mouse Cdk9 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Cdk9 antibody detected the Cdk9 protein (arrow).)

Western Blot (WB) (Mouse Cdk9 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Cdk9 antibody detected the Cdk9 protein (arrow).)
Related Product Information for anti-Cdk9 antibody
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro (By similarity).
Product Categories/Family for anti-Cdk9 antibody
References
Yokoyama, S., et al. Dev. Cell 17(6):836-848(2009). Alarcon, C., et al. Cell 139(4):757-769(2009). Takaya, T., et al. Circ. J. 73(8):1492-1497(2009). Kohoutek, J., et al. Mol. Cell. Biol. 29(12):3280-3285(2009). Elagib, K.E., et al. Blood 112(13):4884-4894(2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,026 Da
NCBI Official Full Name
cyclin-dependent kinase 9
NCBI Official Synonym Full Names
cyclin-dependent kinase 9 (CDC2-related kinase)
NCBI Official Symbol
Cdk9
NCBI Official Synonym Symbols
PITALRE
NCBI Protein Information
cyclin-dependent kinase 9
UniProt Protein Name
Cyclin-dependent kinase 9
Protein Family
UniProt Gene Name
Cdk9
UniProt Entry Name
CDK9_MOUSE

Uniprot Description

CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol.

Protein type: Kinase, protein; EC 2.7.11.22; Nuclear receptor co-regulator; EC 2.7.11.23; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Protein kinase, CMGC; CMGC group; CDK family; CDK/CDK9 subfamily; CDK9 subfamily

Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; membrane; intracellular membrane-bound organelle; cytoplasm; nucleus

Molecular Function: RNA polymerase subunit kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; chromatin binding; kinase activity; transcription factor binding; ATP binding; protein kinase activity

Biological Process: regulation of DNA repair; regulation of histone modification; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of histone phosphorylation; replication fork arrest; positive regulation of transcription from RNA polymerase II promoter; DNA repair; phosphorylation; protein amino acid phosphorylation; response to DNA damage stimulus

Research Articles on Cdk9

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Product Notes

The Cdk9 cdk9 (Catalog #AAA6284523) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cdk9, CT (Cdk9, Cyclin-dependent kinase 9, Cell division protein kinase 9) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cdk9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Cdk9 cdk9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdk9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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