Rabbit anti-Human, Mouse Cdk9 Polyclonal Antibody | anti-Cdk9 antibody

Cdk9, CT (Cdk9, Cyclin-dependent kinase 9, Cell division protein kinase 9) (APC)

Cat. Num. AAA6284524

• Gene Names: Cdk9; PITALRE
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Cdk9; Polyclonal Antibody; CT (Cdk9; Cyclin-dependent kinase 9; Cell division protein kinase 9) (APC); Cell division protein kinase 9; anti-Cdk9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Cdk9 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Cdk9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of Mouse Cdk9.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Cdk9 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Cdk9 antibody detected the Cdk9 protein (arrow).)

Western Blot (WB)

(Mouse Cdk9 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Cdk9 antibody detected the Cdk9 protein (arrow).)

Related Product Information for anti-Cdk9 antibody

Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro (By similarity).

Product Categories/Family for anti-Cdk9 antibody

Antibodies; Protein Kinases

References

Yokoyama, S., et al. Dev. Cell 17(6):836-848(2009). Alarcon, C., et al. Cell 139(4):757-769(2009). Takaya, T., et al. Circ. J. 73(8):1492-1497(2009). Kohoutek, J., et al. Mol. Cell. Biol. 29(12):3280-3285(2009). Elagib, K.E., et al. Blood 112(13):4884-4894(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 18699998
• NCBI GeneID: 107951
• NCBI Accession #: NP_570930.1
• NCBI GenBank Nucleotide #: NM_130860.3
• UniProt Accession #: Q99J95; Q3U002; Q3UMY2; Q3UPT3; Q3UQI6; Q8BTN0; B0R020
• Molecular Weight: 28,026 Da
• NCBI Official Full Name: cyclin-dependent kinase 9
• NCBI Official Synonym Full Names: cyclin-dependent kinase 9 (CDC2-related kinase)
• NCBI Official Symbol: Cdk9
• NCBI Official Synonym Symbols: PITALRE
• NCBI Protein Information: cyclin-dependent kinase 9
• UniProt Protein Name: Cyclin-dependent kinase 9
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk9
• UniProt Entry Name: CDK9_MOUSE

Uniprot Description

CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol.

Protein type: Kinase, protein; EC 2.7.11.22; Nuclear receptor co-regulator; EC 2.7.11.23; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Protein kinase, CMGC; CMGC group; CDK family; CDK/CDK9 subfamily; CDK9 subfamily

Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; membrane; intracellular membrane-bound organelle; cytoplasm; nucleus

Molecular Function: RNA polymerase subunit kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; chromatin binding; kinase activity; transcription factor binding; ATP binding; protein kinase activity

Biological Process: regulation of DNA repair; regulation of histone modification; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of histone phosphorylation; replication fork arrest; positive regulation of transcription from RNA polymerase II promoter; DNA repair; phosphorylation; protein amino acid phosphorylation; response to DNA damage stimulus