Goat anti-Rat Leukocyte Immunoglobulin-Like Receptor C1 Polyclonal Antibody
Leukocyte Immunoglobulin-Like Receptor C1 (LILRC1)
Reactivity
Rat
Applications
ELISA, Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by rat LILRC1 affinity chromatography.
Synonyms
Leukocyte Immunoglobulin-Like Receptor C1; Polyclonal Antibody; Leukocyte Immunoglobulin-Like Receptor C1 (LILRC1); Anti -Leukocyte Immunoglobulin-Like Receptor C1 (LILRC1); anti-Leukocyte Immunoglobulin-Like Receptor C1 antibody
Host
Goat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Selected for its ability to recognize rat LILRC1 in the applications listed.
Purity/Purification
Affinity Purified
Purified by rat LILRC1 affinity chromatography.
Purified by rat LILRC1 affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol.
Applicable Applications for anti-Leukocyte Immunoglobulin-Like Receptor C1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry
Dilution: Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 1.5ng/well. In this format, this antibody shows approximately 30% cross-reactivity with rmLILRCI and less than 1% cross-reactivity with rrLILRC2.
Optimal dilutions should be determined by each laboratory for each application.
Flow Cytometry: Dilute to 50ug/ml and add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome. This antibody has been tested on rat splenocytes for use in flow cytometry.
Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 5ng/lane under non-reducing and reducing conditions.
Dilution: Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 1.5ng/well. In this format, this antibody shows approximately 30% cross-reactivity with rmLILRCI and less than 1% cross-reactivity with rrLILRC2.
Optimal dilutions should be determined by each laboratory for each application.
Flow Cytometry: Dilute to 50ug/ml and add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome. This antibody has been tested on rat splenocytes for use in flow cytometry.
Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 5ng/lane under non-reducing and reducing conditions.
Immunogen
NS0-derived recombinant rat LILRC1 (aa17-248)
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Leukocyte Immunoglobulin-Like Receptor C1 antibody
Leukocyte Immunoglobulin-Like Receptor C1 (rrLILRC1) is a 36kD member of the LILR family. Mature rat LILRC1 is a 279 amino acid type I transmembrane protein with two extracellular lg-like domains and a cytoplasmic tail. The transmembrane region contains an Arg residue, suggesting an association with activating adaptor proteins. LILRC1 is found on B cells, neutrophils and macrophages. Over the region used as immunogen, rat LILRC1 protein is 84% identical to mouse LILRC1 and 58% identical to human LILRA5.
Product Categories/Family for anti-Leukocyte Immunoglobulin-Like Receptor C1 antibody
Similar Products
Product Notes
The Leukocyte Immunoglobulin-Like Receptor C1 (Catalog #AAA613127) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Leukocyte Immunoglobulin-Like Receptor C1 (LILRC1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Leukocyte Immunoglobulin-Like Receptor C1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry Dilution: Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 1.5ng/well. In this format, this antibody shows approximately 30% cross-reactivity with rmLILRCI and less than 1% cross-reactivity with rrLILRC2. Optimal dilutions should be determined by each laboratory for each application. Flow Cytometry: Dilute to 50ug/ml and add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome. This antibody has been tested on rat splenocytes for use in flow cytometry. Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 5ng/lane under non-reducing and reducing conditions. Researchers should empirically determine the suitability of the Leukocyte Immunoglobulin-Like Receptor C1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leukocyte Immunoglobulin-Like Receptor C1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
