Rabbit anti-Human ZIP kinase Polyclonal Antibody | anti-ZIP antibody

ZIP kinase Rabbit Polyclonal

Cat. Num. AAA7605873

• Gene Names: DAPK3; DLK; ZIP; ZIPK
• Reactivity: Human
• Applications: ELISA
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: ZIP kinase; Polyclonal Antibody; ZIP kinase Rabbit Polyclonal; anti-ZIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 454

• Applicable Applications for anti-ZIP antibody: ELISA (EIA)
• Immunogen: Death-associated protein kinase 3
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Western Blot (WB)

(HEK-293 cells were subjected to SDS PAGE followed by western blot with MBS7605873 (ZIP kinase antibody) at dilution of 1:300)

NCBI and Uniprot Product Information

• NCBI GI #: 5162884
• NCBI GeneID: 1613
• Molecular Weight: 37,048 Da
• NCBI Official Full Name: ZIP kinase
• NCBI Official Synonym Full Names: death associated protein kinase 3
• NCBI Official Symbol: DAPK3
• NCBI Official Synonym Symbols: DLK; ZIP; ZIPK
• NCBI Protein Information: death-associated protein kinase 3
• UniProt Protein Name: Death-associated protein kinase 3
• Protein Family: Myosin
• UniProt Gene Name: DAPK3
• UniProt Synonym Gene Names: ZIPK; DAP kinase 3; Dlk; ZIP-kinase
• UniProt Entry Name: DAPK3_HUMAN

NCBI Description

Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

DAPK3: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Monomer and homotrimer. Can also exist as homodimer or form heterodimers with ATF4. Homodimerization is required for activation segment autophosphorylation Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. According to PubMed:17953487, does not interact with PARW. Interacts with AATF, CDC5L, UBE2D1, UBE2D2 AND UBE2D3. Interacts with AR and this interaction is enhanced by AATF. Interacts (via leucine zipper) with TCP10L (via leucine zipper). Interacts (via kinase domain) with DAPK1 (via kinase domain).Interacts with STAT3, NLK and TCF7L2. Isoform 1 and isoform 2 can interact with myosin and PPP1R12A. Isoform 2 is expressed in the bladder smooth muscle. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; Tumor suppressor; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: actin filament; cytoplasm; lipid raft; nucleus; PML body

Molecular Function: ATP binding; cAMP response element binding protein binding; identical protein binding; leucine zipper domain binding; protein binding; protein C-terminus binding; protein homodimerization activity; protein serine/threonine kinase activity; Rho GTPase binding

Biological Process: apoptosis; chromatin modification; cytokinesis; negative regulation of translation; neuron differentiation; positive regulation of apoptosis; positive regulation of cell migration; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of autophagy; regulation of cell shape; regulation of focal adhesion formation; regulation of mitosis; regulation of mitotic cell cycle; regulation of myosin II filament assembly or disassembly; regulation of smooth muscle contraction; regulation of transcription, DNA-dependent; transcription, DNA-dependent