Rabbit Superoxide Dismutase, Mn Polyclonal Antibody | anti-sodA antibody

Superoxide Dismutase, Mn (SOD)

Cat. Num. AAA611036

• Gene Names: sodA; ECK3901; JW3879
• Reactivity: Bovine, Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Superoxide Dismutase; Mn; Polyclonal Antibody; Mn (SOD); Anti -Superoxide Dismutase; anti-sodA antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MnSOD at ~24kD. Does not react with canine. Species Crossreactivity: mouse, rat and bovine
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-sodA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-2ug/ml. Detects MnSOD in RIPA lysates of rat L6 RIPA cells. L6 cell lysates were resolved by electrophoresis and transferred to nitrocellulose probed with S8060-10A. Proteins were visualized using an IgG (HRP) Pab Gt xRb and a chemiluminescence detection system.; Immunohistochemistry(paraffin): 10ug/ml. Detected MnSOD in paraffin embedded rat brain tissue sections.
• Immunogen: Recombinant human MnSOD (manganous superoxide dismutase)
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WB: L6 cell lysate was probed with MBS611036 (0.5ug/ml).)

Related Product Information for anti-sodA antibody

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). The manganese containing 80kD tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain.

Product Categories/Family for anti-sodA antibody

Antibodies; Abs to Superoxide Dismutase

NCBI and Uniprot Product Information

• NCBI GI #: 49176442
• NCBI GeneID: 948403
• NCBI Accession #: NP_418344.3
• NCBI GenBank Nucleotide #: NC_000913.2
• UniProt Accession #: P00448; Q2M8K5
• Molecular Weight: 23,097 Da
• NCBI Official Full Name: superoxide dismutase, Mn
• NCBI Official Symbol: sodA
• NCBI Official Synonym Symbols: ECK3901; JW3879
• NCBI Protein Information: superoxide dismutase, Mn
• UniProt Protein Name: Superoxide dismutase [Mn]
• Protein Family: Superoxide dismutase
• UniProt Gene Name: sodA
• UniProt Entry Name: SODM_ECOLI

NCBI Description

SoxRS regulon. [More information is available at EcoGene: EG10953].

Uniprot Description

Function: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2.

Cofactor: Binds 1 manganese ion per subunit.

Subunit structure: Homodimer.

Sequence similarities: Belongs to the iron/manganese superoxide dismutase family.