Western Blot (WB)
(Western Blot analysis of (1) HeLa cell lysate and (2) rat brain tissue extract using MBS610841)
Rabbit Superoxide Dismutase, Mn Polyclonal Antibody | anti-sodA antibody
Superoxide Dismutase, Mn (SOD Mn)
Gene Names
sodA; ECK3901; JW3879
Reactivity
Bovine, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Superoxide Dismutase; Mn; Polyclonal Antibody; Mn (SOD Mn); Anti -Superoxide Dismutase; anti-sodA antibody
Host
Rabbit
Reactivity
Bovine, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes a rat ~25kD protein, corresponding to the apparent molecular mass of rat Mn superoxide dismutase (SOD). Species Crossreactivity: human, mouse, bovine, canine, chicken, Drosophila, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.0, 15mg/ml BSA, 0.1% sodium azide, 50% glycerol.
Applicable Applications for anti-sodA antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Dilution: Western Blot (Colorimetric) (8): 0.5ug/ml
Flow Cytometry: 10ug/ml.
Optimal dilutions to be determined by researcher.
Positive Control:
Rat Brain Tissue Extract
Flow Cytometry: 10ug/ml.
Optimal dilutions to be determined by researcher.
Positive Control:
Rat Brain Tissue Extract
Immunogen
Rat Mn SOD8.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis of (1) HeLa cell lysate and (2) rat brain tissue extract using MBS610841)
Western Blot (WB)
(Western Blot analysis of (1) HeLa cell lysate and (2) rat brain tissue extract using MBS610841)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of U251 cells using MBS610841 (right) and control rabbit IgG (left).)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of U251 cells using MBS610841 (right) and control rabbit IgG (left).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of human skeletal muscle tissue stained with MBS610841 (10ug/ml).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of human skeletal muscle tissue stained with MBS610841 (10ug/ml).)
Related Product Information for anti-sodA antibody
Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32kD dimer in the cytosol. Mn SOD is an 80kD tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from TNF-mediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDA-and nitric oxide-induced neurotoxicity (5).
Product Categories/Family for anti-sodA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,097 Da
NCBI Official Full Name
superoxide dismutase, Mn
NCBI Official Symbol
sodA
NCBI Official Synonym Symbols
ECK3901; JW3879
NCBI Protein Information
superoxide dismutase, Mn
UniProt Protein Name
Superoxide dismutase [Mn]
Protein Family
UniProt Gene Name
sodA
UniProt Entry Name
SODM_ECOLI
NCBI Description
SoxRS regulon. [More information is available at EcoGene: EG10953].
Uniprot Description
Function: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2.
Cofactor: Binds 1 manganese ion per subunit.
Subunit structure: Homodimer.
Sequence similarities: Belongs to the iron/manganese superoxide dismutase family.
Research Articles on sodA
Similar Products
Product Notes
The sodA soda (Catalog #AAA610841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Superoxide Dismutase, Mn (SOD Mn) reacts with Bovine, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Superoxide Dismutase, Mn can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). Dilution: Western Blot (Colorimetric) (8): 0.5ug/ml Flow Cytometry: 10ug/ml. Optimal dilutions to be determined by researcher. Positive Control: Rat Brain Tissue Extract. Researchers should empirically determine the suitability of the sodA soda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase, Mn, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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