Typical Testing Data/Standard Curve (for reference only)
Human Carbonic Anhydrase 12 (CA12) ELISA Kit | CA12 elisa kit
Human Carbonic Anhydrase 12 (CA12) ELISA Kit
Gene Names
CA12; CAXII; CA-XII; T18816; HsT18816
Reactivity
Human
Synonyms
Carbonic Anhydrase 12 (CA12); Human Carbonic Anhydrase 12 (CA12) ELISA Kit; CA12 elisa kit
Reactivity
Human
Sequence Length
354
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05mg/ml - 10mg/ml
Sensitivity
0.02mg/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CA12 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Carbonic Anhydrase 12 (also known as CA12) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CA12 antibody. CA12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CA12 Antibody is added and binds to CA12 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CA12 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CA12. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CA12 antibody. CA12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CA12 Antibody is added and binds to CA12 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CA12 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CA12. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,408 Da
NCBI Official Full Name
carbonic anhydrase 12 isoform 1
NCBI Official Synonym Full Names
carbonic anhydrase 12
NCBI Official Symbol
CA12
NCBI Official Synonym Symbols
CAXII; CA-XII; T18816; HsT18816
NCBI Protein Information
carbonic anhydrase 12
UniProt Protein Name
Carbonic anhydrase 12
Protein Family
UniProt Gene Name
CA12
UniProt Synonym Gene Names
CA-XII
NCBI Description
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]
Uniprot Description
Reversible hydration of carbon dioxide.
Research Articles on CA12
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Product Notes
The Human CA12 ca12 (Catalog #AAA167259) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA167259 ELISA Kit recognizes Human CA12. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase 12 (CA12), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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