Immunocytochemistry (ICC)
(Immunocytochemical staining of HeLa cells using MBS606116. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.)
Mouse anti-Human Basonuclin 2 Monoclonal Antibody | anti-BNC2 antibody
Basonuclin 2 (Basonuclin-2, Basonuclin2, BNC2, Zinc Finger Protein Basonuclin 2)
Gene Names
BNC2; BSN2; FLJ20043; FLJ34928; DKFZp686A01127; BNC2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Basonuclin 2; Monoclonal Antibody; Basonuclin 2 (Basonuclin-2; Basonuclin2; BNC2; Zinc Finger Protein Basonuclin 2); Anti -Basonuclin 2 (Basonuclin-2; anti-BNC2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9j19
Specificity
Recognizes human BNC2.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 1% BSA and 0.05% sodium azide.
Applicable Applications for anti-BNC2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Immunocytochemistry, Western Blot and Dot Blot.
Dilution: Immunocytochemistry: 2-100ug/ml
Dilution: Immunocytochemistry: 2-100ug/ml
Immunogen
Synthetic peptide corresponding to the middle region of human BNC2
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunocytochemistry (ICC)
(Immunocytochemical staining of HeLa cells using MBS606116. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.)
Immunocytochemistry (ICC)
(Immunocytochemical staining of HeLa cells using MBS606116. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.)
Western Blot (WB)
(Western Blot analysis using the recombinant immunogen (not full-length protein) as sample.)
Western Blot (WB)
(Western Blot analysis using the recombinant immunogen (not full-length protein) as sample.)
Related Product Information for anti-BNC2 antibody
Basonuclin 2 is a probable transcription factor specific for skin keratinocytes. It may play a role in the differentiation of spermatozoa and oocytes. Basonuclin 2 is highly expressed in testis, uterus and small intestine, and weakly expressed in colon and prostate. It is also expressed in skin, primary keratinocytes, immortalized keratinocytes, and HeLa and HEK293 cells. It is not detected in blood, thymus, spleen or HepG2 cells.
Product Categories/Family for anti-BNC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
45,374 Da
NCBI Official Full Name
basonuclin 2
NCBI Official Synonym Full Names
basonuclin 2
NCBI Official Symbol
BNC2
NCBI Official Synonym Symbols
BSN2; FLJ20043; FLJ34928; DKFZp686A01127; BNC2
NCBI Protein Information
zinc finger protein basonuclin-2; OTTHUMP00000126958
UniProt Protein Name
Basonuclin 2
Protein Family
UniProt Gene Name
BNC2
UniProt Entry Name
Q06HC1_HUMAN
Research Articles on BNC2
1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
2. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
3. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
4. Loss of BNC2 is associated with esophageal adenocarcinoma.
5. transcripts are initiated from six promoters, are alternatively spliced at multiple positions, and are polyadenylated at four sites
6. the extreme conservation of the basonuclin 2 amino acid sequence across vertebrates suggests that basonuclin 2 serves an important function, presumably as a regulatory protein of DNA transcription
7. molecular cloning
8. Data show that basonuclin (Bn)2 but not bn1 colocalizes with SC35 in nuclear speckles, and may have a function in nuclear processing of mRNA.
2. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
3. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
4. Loss of BNC2 is associated with esophageal adenocarcinoma.
5. transcripts are initiated from six promoters, are alternatively spliced at multiple positions, and are polyadenylated at four sites
6. the extreme conservation of the basonuclin 2 amino acid sequence across vertebrates suggests that basonuclin 2 serves an important function, presumably as a regulatory protein of DNA transcription
7. molecular cloning
8. Data show that basonuclin (Bn)2 but not bn1 colocalizes with SC35 in nuclear speckles, and may have a function in nuclear processing of mRNA.
Similar Products
Product Notes
The BNC2 bnc2 (Catalog #AAA606116) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Basonuclin 2 (Basonuclin-2, Basonuclin2, BNC2, Zinc Finger Protein Basonuclin 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Basonuclin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA). Suitable for use in Immunocytochemistry, Western Blot and Dot Blot. Dilution: Immunocytochemistry: 2-100ug/ml. Researchers should empirically determine the suitability of the BNC2 bnc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Basonuclin 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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