Western Blot (WB)
(Western Blot detection against Immunogen (37.51kD).)
Mouse anti-Human BNC1 Monoclonal Antibody
BNC1 (Zinc Finger Protein Basonuclin-1, BNC)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
BNC1; Monoclonal Antibody; BNC1 (Zinc Finger Protein Basonuclin-1; BNC); Anti -BNC1 (Zinc Finger Protein Basonuclin-1; anti-BNC1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1F4
Specificity
Recognizes human BNC1.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
GKPFPEGERPCHRESVIESSGAISQTPEQATHNSERETEHTPALIMVPREVEDGGHEHYFTPGMEPQVPFSDYMELQQRLLAGGLFSALSNRGMAFPCLEDSKELEH
Applicable Applications for anti-BNC1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Immunogen
Partial recombinant corresponding to aa599-705 from human BNC1 (NP_001708) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (37.51kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37.51kD).)
Testing Data
(Detection limit for recombinant GST tagged BNC1 is 0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged BNC1 is 0.3ng/ml as a capture antibody.)
Related Product Information for anti-BNC1 antibody
Bnc 1 is a 994aa transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. It is a zinc finger protein with three separated pairs of zinc fingers and a nuclear localization signal. Bnc 1 is a soluble protein that can shuttle between the nucleus and the cytoplasm, and its location depends on the proliferative potential of the cell. It is expressed relatively uniformly in the nucleoplasm, and phosphorylation on Ser-537 and Ser-541 leads to its cytoplasmic localization. It is present in the basal cell layer of the epidermis, in hair follicles and also in abundance in the germ cells of testis and ovary, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart. Reports suggest that it plays a regulatory role in keratinocyte proliferation and also in rRNA transcription. It is also known to play a role in the differentiation of spermatozoa and oocytes.
Product Categories/Family for anti-BNC1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The BNC1 (Catalog #AAA6005672) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BNC1 (Zinc Finger Protein Basonuclin-1, BNC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BNC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the BNC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GKPFPEGERP CHRESVIESS GAISQTPEQA THNSERETEH TPALIMVPRE VEDGGHEHYF TPGMEPQVPF SDYMELQQRL LAGGLFSALS NRGMAFPCLE DSKELEH. It is sometimes possible for the material contained within the vial of "BNC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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