Rabbit anti-Human WHSC1L1 Polyclonal Antibody | anti-WHSC1L1 antibody

WHSC1L1, NT (WHSC1L1, NSD3, Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, Wolf-Hirschhorn syndrome candidate 1-like protein 1) (AP)

Cat. Num. AAA6363577

• Gene Names: WHSC1L1; NSD3; pp14328
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: WHSC1L1; Polyclonal Antibody; NT (WHSC1L1; NSD3; Histone-lysine N-methyltransferase NSD3; Nuclear SET domain-containing protein 3; Protein whistle; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; Wolf-Hirschhorn syndrome candidate 1-like protein 1) (AP); Wolf-Hirschhorn syndrome candidate 1-like protein 1; anti-WHSC1L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-WHSC1L1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: WHSC1L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the N-terminal region of human WHSC1L1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of WHSC1L1 (arrow) using rabbit polyclonal WHSC1L1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the WHSC1L1 gene.)

Related Product Information for anti-WHSC1L1 antibody

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described.

Product Categories/Family for anti-WHSC1L1 antibody

Antibodies; Enzymes; Methyltransferase

References

Zhou, Z., et al. Biochem. Biophys. Res. Commun. 398(3):565-570(2010). Kim, S.M., et al. Biochem. Biophys. Res. Commun. 345(1):318-323(2006). Tonon, G., et al. Proc. Natl. Acad. Sci. U.S.A. 102(27):9625-9630(2005). Rosati, R., et al. Blood 99(10):3857-3860(2002). Stec, I., et al. Genomics 76 (1-3), 5-8 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 13699813
• NCBI GeneID: 54904
• NCBI Accession #: NP_060248.2; NP_075447.1
• NCBI GenBank Nucleotide #: NM_017778.2
• UniProt Accession #: Q9BZ95; Q1RMD3; Q3B796; Q6ZSA5; Q9BYU8; Q9BYU9; Q9H2M8; Q9H9W9; Q9NXA6; B7ZL11; D3DSX1
• Molecular Weight: 160,314 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase NSD3 isoform short
• NCBI Official Synonym Full Names: Wolf-Hirschhorn syndrome candidate 1-like 1
• NCBI Official Symbol: WHSC1L1
• NCBI Official Synonym Symbols: NSD3; pp14328
• NCBI Protein Information: histone-lysine N-methyltransferase NSD3; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; WHSC1-like protein 1; nuclear SET domain-containing protein 3; protein whistle
• UniProt Protein Name: Histone-lysine N-methyltransferase NSD3
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: WHSC1L1
• UniProt Synonym Gene Names: NSD3; WHSC1-like protein 1
• UniProt Entry Name: NSD3_HUMAN

NCBI Description

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

WHSC1L1: is related to the Wolf-Hirschhorn syndrome candidate-1 protein containing PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced isoforms have been described.

Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; Oncoprotein; Histone-binding; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: nucleoplasm; chromosome; nucleus

Molecular Function: protein binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; histone H3-K4 methylation; negative regulation of transcription, DNA-dependent

Disease: Leukemia, Acute Myeloid