Western Blot (WB)
(Western Blot of recombinant Twist using MBS602939)
Mouse TWIST1 Monoclonal Antibody | anti-TWIST1 antibody
TWIST1 (Twist Homolog 1 (Drosophila), ACS3, bHLHa38, BPES2, BPES3, H-twist, SCS, Twist-related Protein 1)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Western Blot: 0.2-2ug/ml
Immunocytochemistry: 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with T9300-02.
Flow Cytometry: 0.5-2ug. HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with T9300-02 or isotype control followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.
Western Blot (WB)
(Western Blot of recombinant Twist using MBS602939)
Western Blot (WB)
(Western Blot of recombinant Twist using MBS602939)
Testing Data
(Whole cell lysate from human A2058 (25ug) , mouse NIH3T3 (25ug) and rat F2408 (25ug) cells using MBS602939. Predicted molecular weight: 20kD)
Testing Data
(Whole cell lysate from human A2058 (25ug) , mouse NIH3T3 (25ug) and rat F2408 (25ug) cells using MBS602939. Predicted molecular weight: 20kD)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained MBS602939 (shaded) or isotype control (unshaded) followed by IgG (Alexa Fluor® 488) goat anti-mouse.)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained MBS602939 (shaded) or isotype control (unshaded) followed by IgG (Alexa Fluor® 488) goat anti-mouse.)
Testing Data
(HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS602939.)
Testing Data
(HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS602939.)
NCBI and Uniprot Product Information
NCBI Description
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq]
Uniprot Description
TWIST1: Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed. Subset of mesodermal cells.
Protein type: Transcription regulation; Cell development/differentiation; DNA-binding; Inhibitor
Chromosomal Location of Human Ortholog: 7p21.2
Cellular Component: nucleus
Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; protein heterodimerization activity; bHLH transcription factor binding; transcription factor binding
Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; negative regulation of skeletal muscle development; muscle development; rhythmic process; neuron migration; palate development; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of histone phosphorylation; negative regulation of histone acetylation; odontogenesis; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of osteoblast differentiation; ossification; in utero embryonic development; negative regulation of transcription factor activity; outer ear morphogenesis; negative regulation of tumor necrosis factor production; embryonic cranial skeleton morphogenesis; positive regulation of tumor necrosis factor production; positive regulation of fatty acid beta-oxidation; osteoblast differentiation; positive regulation of angiogenesis; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; regulation of bone mineralization; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of phosphoinositide 3-kinase cascade
Disease: Robinow-sorauf Syndrome; Craniosynostosis 1; Saethre-chotzen Syndrome
Research Articles on TWIST1
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Product Notes
The TWIST1 twist1 (Catalog #AAA602939) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TWIST1 (Twist Homolog 1 (Drosophila), ACS3, bHLHa38, BPES2, BPES3, H-twist, SCS, Twist-related Protein 1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TWIST1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Gel Shift Assay (GS/EMSA). Suitable for use in ELISA, Western Blot, Dot Blot, Flow Cytometry and Immunocytochemistry. Dilution: Western Blot: 0.2-2ug/ml Immunocytochemistry: 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with T9300-02. Flow Cytometry: 0.5-2ug. HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with T9300-02 or isotype control followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG. Researchers should empirically determine the suitability of the TWIST1 twist1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TWIST1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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