Mouse DDX17 Monoclonal Antibody | anti-DDX17 antibody

DDX17 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 17, DKFZp761H2016, RH70)

Cat. Num. AAA602926

• Gene Names: DDX17; P72; RH70; DKFZp761H2016; DDX17
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: DDX17; Monoclonal Antibody; DDX17 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 17; DKFZp761H2016; RH70); Anti -DDX17 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 17; anti-DDX17 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8J107
• Specificity: Recognizes human DDX17. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 1% BSA and 0.05% sodium azide.

• Applicable Applications for anti-DDX17 antibody: Western Blot (WB), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Immunocytochemistry, Dot Blot and Western Blot.; Dilution: Western Blot: 0.2-2ug/ml
• Immunogen: Synthetic peptide near the C-terminus of human DDX17.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of human DDX17 by Western blot uksing MBS602926. Samples: lane 1: Whole cell lysate from human HEK293 (H, 25ug), Lane 2: mouse NIH3T3 (25ug) and Lane 3: rat F2408 (25ug) cells.)

Testing Data

(Staining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS602926.)

Testing Data

Related Product Information for anti-DDX17 antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family.

Product Categories/Family for anti-DDX17 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 47678395
• NCBI GeneID: 10521
• UniProt Accession #: Q92841; Q69YT1; Q6ICD6; B1AHM0; Q59F66; Q9UQL5
• Molecular Weight: 72,371 Da
• NCBI Official Full Name: DDX17
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
• NCBI Official Symbol: DDX17
• NCBI Official Synonym Symbols: P72; RH70; DKFZp761H2016; DDX17
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX17; DEAD/H box 17; OTTHUMP00000199226; DEAD box protein 17; DEAD box protein p72; RNA-dependent helicase p72; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD)
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX17
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX17
• UniProt Entry Name: DDX17_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. [provided by RefSeq]

Uniprot Description

DDX17: RNA-dependent ATPase activity. Involved in transcriptional regulation. Transcriptional coactivator for estrogen receptor ESR1. Increases ESR1 AF-1 domain-mediated transactivation. Synergizes with DDX5 and SRA1 RNA to activate MYOD1 transcriptional activity and probably involved in skeletal muscle differentiation. Required for zinc-finger antiviral protein ZC3HAV1-mediated mRNA degradation. Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; Nucleolus; Spliceosome; RNA-binding; Nuclear receptor co-regulator; EC 3.6.4.13; Helicase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; membrane; nucleolus; nucleus

Molecular Function: protein binding; RNA-dependent ATPase activity; RNA binding; transcription coactivator activity; estrogen receptor binding; ATP binding; RNA helicase activity

Biological Process: RNA processing; transcription, DNA-dependent; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter