Mouse VAMP 1,2 Monoclonal Antibody | anti-VAMP1 antibody

VAMP 1,2 (Vesicle-Associated Membrane Protein, Synaptobrevin)

Cat. Num. AAA604808

• Gene Names: VAMP1; SYB1; VAMP-1; DKFZp686H12131
• Reactivity: Bovine, Hamster, Human, Mouse, Porcine, Rat, Xenopus
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate pecipitation.
• Synonyms: VAMP 1; 2; Monoclonal Antibody; 2 (Vesicle-Associated Membrane Protein; Synaptobrevin); Anti -VAMP 1; anti-VAMP1 antibody

• Host: Mouse
• Reactivity: Bovine, Hamster, Human, Mouse, Porcine, Rat, Xenopus
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 4E240
• Specificity: Recognizes human VAMP on SDS-PAGE immunoblots at ~18kD. Recognizes the VAMP1 aa1-118 and the VAMP2 aa33-96-GST fusion proteins expressed in E. coli. No reactivity was seen with a ~18kD protein in mouse liver extracts. Species Crossreactivity: mouse, rat, bovine, hamster, porcine and Xenopus.
• Purity/Purification: Purified; Purified by ammonium sulfate pecipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.

• Applicable Applications for anti-VAMP1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot (Colorimetric): 1:4000; Immunohistochemistry: 1:50 on human neuroblastoma tissue.; Optimal dilutions to be determined by researcher.
• Immunogen: Immunoprecipitated synaptic vesicle-containing fractions from human brain homogenates.
• Positive Control: Rat Brain Tissue Extract
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse brain tissue extract using MBS604808.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of human neuroblastoma tissue using MBS604808 (1:50).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of Xenopus embryonic brain, using MBS604808..)

Related Product Information for anti-VAMP1 antibody

Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18kD integral membrane protein localized to the cytoplasmic surface of synaptic vesicle (2). VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxyl-terminal tail (3). Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system (3). VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons associated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells (4). Evidences for VAMP's involvement in vesicular transport and neurotransmission include the requirement of its yeast homologs, SNC 1 and 2, in targeting and fusion of Golgi- derived vesicles with the plasma membrane (5). VAMP is also the target of clostridial neurotoxins that block neurotransmitter release from presynaptic terminals (6). VAMP is a component of a 20S complex implicated in vesicle docking and fusion. The formation of 20S complex involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF (7).

Product Categories/Family for anti-VAMP1 antibody

Antibodies; Abs to Membrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 40549446
• NCBI GeneID: 6843
• NCBI Accession #: NP_954740.1
• NCBI GenBank Nucleotide #: NM_199245.1
• UniProt Accession #: P23763; O75468; Q15857; Q6FG94; Q8IVC9; A8MVP3; D3DUR3
• Molecular Weight: 12,902 Da
• NCBI Official Full Name: vesicle-associated membrane protein 1 isoform 2
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 1 (synaptobrevin 1)
• NCBI Official Symbol: VAMP1
• NCBI Official Synonym Symbols: SYB1; VAMP-1; DKFZp686H12131
• NCBI Protein Information: vesicle-associated membrane protein 1; synaptobrevin 1; synaptobrevin-1; OTTHUMP00000238453; OTTHUMP00000238454; OTTHUMP00000238455; OTTHUMP00000238560; OTTHUMP00000238561
• UniProt Protein Name: Vesicle-associated membrane protein 1
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP1
• UniProt Synonym Gene Names: SYB1
• UniProt Entry Name: VAMP1_HUMAN

NCBI Description

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq]

Uniprot Description

VAMP1: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p

Cellular Component: SNARE complex; synaptic vesicle; mitochondrial outer membrane; neuron projection; cell surface; synaptic vesicle membrane; integral to plasma membrane; cell junction; cytosol

Molecular Function: SNAP receptor activity; protein binding; SNARE binding

Biological Process: vesicle fusion; exocytosis; pathogenesis

Disease: Spastic Ataxia 1, Autosomal Dominant