Rabbit CDCA3 Polyclonal Antibody | anti-CDCA3 antibody

CDCA3 Antibody

Cat. Num. AAA9605001

• Gene Names: CDCA3; GRCC8; TOME-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CDCA3; Polyclonal Antibody; CDCA3 Antibody; C8; CDCA 3; Cell division cycle associated 3; Cell division cycle associated protein 3; Gene rich cluster C8; Gene rich cluster protein C8; GRCC 8; GRCC8; MGC2577; TOME 1; TOME1; Trigger of mitotic entry 1; Trigger of mitotic entry protein 1; anti-CDCA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CDCA3 antibody detects endogenous levels of total CDCA3
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 219

• Applicable Applications for anti-CDCA3 antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Cytoplasmic. Cytosol.
• Predicted Cross Reactivity: Bovine, Horse, Sheep, Rabbit, Dog
• Similarity: Bovine (82%), Horse (100%), Sheep (91%), Rabbit (100%), Dog (82%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunofluorescence (IF)

(MBS9605001 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using CDCA3 antibody.)

Immunohistochemistry (IHC)

(MBS9605001 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-CDCA3 antibody

Function: F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity).
Subunit Structure: Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity).
Post-translational Modifications: Ubiquitinated and degraded by the APC/C-Cdh1 complex.
Similarity: The KEN box is required for the association with the APC/C-Cdh1 complex.

NCBI and Uniprot Product Information

• NCBI GI #: 662236834
• NCBI GeneID: 83461
• NCBI Accession #: NP_001284532.1
• NCBI GenBank Nucleotide #: NM_001297603.2
• UniProt Accession #: Q99618; A8K5V6; D3DUS6
• Molecular Weight: Observed: 28 kD; Predicted: 29 kDa
• NCBI Official Full Name: cell division cycle-associated protein 3 isoform 3
• NCBI Official Synonym Full Names: cell division cycle associated 3
• NCBI Official Symbol: CDCA3
• NCBI Official Synonym Symbols: GRCC8; TOME-1
• NCBI Protein Information: cell division cycle-associated protein 3
• UniProt Protein Name: Cell division cycle-associated protein 3
• Protein Family: Cell division cycle-associated protein
• UniProt Gene Name: CDCA3
• UniProt Synonym Gene Names: C8; GRCC8; TOME1; TOME-1

Uniprot Description

F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase ().