Mouse anti-Human HLA Class 2 Antigen DQ6 Monoclonal Antibody | anti-MICA antibody

HLA Class 2 Antigen DQ6 (IgM, X-Reactive)

Cat. Num. AAA603749

• Gene Names: MICA; MIC-A; PERB11.1
• Reactivity: Human
• Purity: Ascites; Ascites
• Synonyms: HLA Class 2 Antigen DQ6; Monoclonal Antibody; HLA Class 2 Antigen DQ6 (IgM; X-Reactive); Anti -HLA Class 2 Antigen DQ6 (IgM; anti-MICA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 4i147
• Specificity: Reacts to HLA Class 2 Antigen-DQ6. Additional crossreactivity occurs with related proteins.. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute to 100ul sterile ddH2O to make a 10X stock solution.

• Application Notes: Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, and Chimerism Studies.; Dilution: Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution.
• Immunogen: B cells expressing HLA class 2 antigen
• Cytotoxicity: Expected Results:; Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.; Limitations:; Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-MICA antibody

Antibodies; Abs to HLA

NCBI and Uniprot Product Information

• NCBI GI #: 557559
• NCBI GeneID: 100507436
• UniProt Accession #: Q29983; O60917; O60918; O60919; O60920; O60933; O77956; O78150; A8KQR0; A9LRW5; A9QVE0; B1VJF1
• Molecular Weight: 42,915 Da
• NCBI Official Full Name: HLA class I alpha chain, partial
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence A
• NCBI Official Symbol: MICA
• NCBI Official Synonym Symbols: MIC-A; PERB11.1
• NCBI Protein Information: MHC class I polypeptide-related sequence A; HLA class I antigen; stress inducible class I homolog; MHC class I chain-related protein A
• UniProt Protein Name: MHC class I polypeptide-related sequence A
• Protein Family: Lantibiotic michiganin
• UniProt Gene Name: MICA
• UniProt Synonym Gene Names: PERB11.1; MIC-A
• UniProt Entry Name: MICA_HUMAN

NCBI Description

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

MICA: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma. Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the MHC class I family. MIC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: extracellular space; cell surface; integral to plasma membrane; cytoplasm

Molecular Function: protein binding; beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; viral reproduction; response to heat; natural killer cell mediated cytotoxicity; cytolysis; defense response to bacterium; immune response to tumor cell; gamma-delta T cell activation; response to DNA damage stimulus; defense response to virus; T cell mediated cytotoxicity

Disease: Psoriatic Arthritis, Susceptibility To; Psoriasis 1, Susceptibility To