Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PTPRG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human, Mouse PTPRG Polyclonal Antibody | anti-PTPRG antibody
PTPRG Polyclonal Antibody
Gene Names
PTPRG; PTPG; HPTPG; RPTPG; R-PTP-GAMMA
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
PTPRG; Polyclonal Antibody; PTPRG Polyclonal Antibody; HPTPG; PTPG; R-PTP-GAMMA; RPTPG; anti-PTPRG antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
YRYTGSLTTPPCSEIVEWIVFRRPVPISYHQLEAFYSIFTTEQQDHVKSVEYLRNNFRPQQRLHDRVVSKSAVRDSWNHDMTDFLENPLGTEASKVCSSPPIHMKVQPLNQTALQVSWSQPETIYHPPIMNYMISYSWTKNEDEKEKTFTKDSDKDLKATISHVSPDSLYLFRVQAVCRNDMRSDFSQTMLFQANTTRIFQ
Sequence Length
1445
Applicable Applications for anti-PTPRG antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human PTPRG
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Membrane, Single-pass type I membrane protein
Positive Samples
A-549, 293T, Mouse kidney, Mouse heart, Mouse liver, Rat spleen
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PTPRG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PTPRG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-PTPRG antibody
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this PTP contains a carbonic anhydrase-like (CAH) domain, which is also found in the extracellular region of PTPRBETA/ZETA. This gene is located in a chromosomal region that is frequently deleted in renal cell carcinoma and lung carcinoma, thus is thought to be a candidate tumor suppressor gene.
Product Categories/Family for anti-PTPRG antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 158kDa; 162kDa
Observed: 162kDa
Observed: 162kDa
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase gamma
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type G
NCBI Official Symbol
PTPRG
NCBI Official Synonym Symbols
PTPG; HPTPG; RPTPG; R-PTP-GAMMA
NCBI Protein Information
receptor-type tyrosine-protein phosphatase gamma
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase gamma
Protein Family
UniProt Gene Name
PTPRG
UniProt Synonym Gene Names
PTPG; Protein-tyrosine phosphatase gamma; R-PTP-gamma
NCBI Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this PTP contains a carbonic anhydrase-like (CAH) domain, which is also found in the extracellular region of PTPRBETA/ZETA. This gene is located in a chromosomal region that is frequently deleted in renal cell carcinoma and lung carcinoma, thus is thought to be a candidate tumor suppressor gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Possesses tyrosine phosphatase activity.
Research Articles on PTPRG
Similar Products
Product Notes
The PTPRG ptprg (Catalog #AAA9134259) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTPRG Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PTPRG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the PTPRG ptprg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: YRYTGSLTTP PCSEIVEWIV FRRPVPISYH QLEAFYSIFT TEQQDHVKSV EYLRNNFRPQ QRLHDRVVSK SAVRDSWNHD MTDFLENPLG TEASKVCSSP PIHMKVQPLN QTALQVSWSQ PETIYHPPIM NYMISYSWTK NEDEKEKTFT KDSDKDLKAT ISHVSPDSLY LFRVQAVCRN DMRSDFSQTM LFQANTTRIF Q. It is sometimes possible for the material contained within the vial of "PTPRG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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