Human MHC class I polypeptide-related sequence A ELISA Kit | MICA elisa kit

Human MHC class I polypeptide-related sequence A ELISA Kit

Cat. Num. AAA762924

• Gene Names: MICA; MIC-A; PERB11.1
• Reactivity: Human
• Synonyms: MHC class I polypeptide-related sequence A; Human MHC class I polypeptide-related sequence A ELISA Kit; MICA/MIC-A; MICA elisa kit

• Reactivity: Human
• Sequence Length: 359

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MICA elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MICAantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MICA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MICA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MICAcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 27544412
• NCBI GeneID: 100507436
• Molecular Weight: 31,577 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence A, partial
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence A
• NCBI Official Symbol: MICA
• NCBI Official Synonym Symbols: MIC-A; PERB11.1
• NCBI Protein Information: MHC class I polypeptide-related sequence A
• UniProt Protein Name: MHC class I polypeptide-related sequence A
• Protein Family: Microperfuranone synthase
• UniProt Gene Name: MICA
• UniProt Synonym Gene Names: MIC-A
• UniProt Entry Name: MICA_HUMAN

NCBI Description

This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

MICA: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma. Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the MHC class I family. MIC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: cell surface; cytoplasm; extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: antigen binding; beta-2-microglobulin binding; natural killer cell lectin-like receptor binding; protein binding

Biological Process: antigen processing and presentation; cytolysis; defense response to bacterium; defense response to virus; gamma-delta T cell activation; immune response to tumor cell; natural killer cell mediated cytotoxicity; negative regulation of natural killer cell activation; negative regulation of natural killer cell mediated cytotoxicity; regulation of immune response; response to DNA damage stimulus; response to heat; T cell mediated cytotoxicity; viral reproduction

Disease: Psoriasis 1, Susceptibility To; Psoriatic Arthritis, Susceptibility To