Rabbit MHC class I polypeptide-related sequence A ELISA Kit | MICA elisa kit

Rabbit MHC class I polypeptide-related sequence A ELISA Kit

Cat. Num. AAA729919

• Gene Names: MICA; MIC-A; PERB11.1
• Reactivity: Rabbit
• Synonyms: MHC class I polypeptide-related sequence A; Rabbit MHC class I polypeptide-related sequence A ELISA Kit; MICA elisa kit

• Reactivity: Rabbit

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MICA elisa kit

Intended Uses: This MHC I/H-2 II ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of MHC I/H-2 II in the sample, thisMHC I/H-2 II ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusMHC I/H-2 II concentration. The concentration of MHC I/H-2 II in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This MHC I/H-2 II enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for MHC I/H-2 II. Standards or samples are then added to the microtiter plate wells and MHC I/H-2 II if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofMHC I/H-2 II present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MHC I/H-2 II are added to each well to "sandwich" the MHC I/H-2 II immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containMHC I/H-2 II and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for MICA elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 294660768
• NCBI GeneID: 100507436
• NCBI Accession #: NP_001170990.1
• NCBI GenBank Nucleotide #: NM_001177519.2
• Molecular Weight: 31,577 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence A isoform 2 (MICA*00801)
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence A
• NCBI Official Symbol: MICA
• NCBI Official Synonym Symbols: MIC-A; PERB11.1
• NCBI Protein Information: MHC class I polypeptide-related sequence A; HLA class I antigen; stress inducible class I homolog; MHC class I chain-related protein A
• UniProt Protein Name: MHC class I polypeptide-related sequence A
• Protein Family: Microperfuranone synthase
• UniProt Gene Name: MICA
• UniProt Synonym Gene Names: PERB11.1; MIC-A
• UniProt Entry Name: MICA_HUMAN

NCBI Description

This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

MICA: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma. Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the MHC class I family. MIC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: extracellular space; cell surface; integral to plasma membrane; cytoplasm

Molecular Function: protein binding; beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; viral reproduction; natural killer cell mediated cytotoxicity; response to heat; cytolysis; defense response to bacterium; immune response to tumor cell; defense response to virus; gamma-delta T cell activation; response to DNA damage stimulus; T cell mediated cytotoxicity

Disease: Psoriatic Arthritis, Susceptibility To; Psoriasis 1, Susceptibility To