GPC3 recombinant protein

Recombinant Human GPC3 protein, C-His Tag

Cat. Num. AAA1560039

• Gene Names: GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: GPC3; Recombinant Human GPC3 protein; C-His Tag; OCI5; Glypican-3; GTR2-2; MXR7; DGSX; SDYS; SGB; SGBS; SGBS1; GPC3 recombinant protein

• Host: Mammalian cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Source: Human
• Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
• Construction: A DNA sequence encoding the human GPC3 (Met1-His559) was fused with the C-terminal His Tag
• Preparation and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for ~12 months from the date of receipt.; Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise

SDS-Page

Product Categories/Family for GPC3 recombinant protein

Protein

References

Liu, Xu, Han, Wan, Zheng, Li, Liao, Xiao, Zhong, Zheng, Wang, Li, Chen, Wei, Wang (2020) LRCH1 deficiency enhances LAT signalosome formation and CD8+ T cell responses against tumors and pathogens Proceedings of the National Academy of Sciences of the United States of America

NCBI and Uniprot Product Information

• NCBI GI #: 257471010
• NCBI GeneID: 2719
• NCBI Accession #: NP_001158091.1
• NCBI GenBank Nucleotide #: NM_001164619.1
• UniProt Accession #: P51654; Q2L880; Q2L882; C9JLE3; G3V1R0
• Molecular Weight: 65,563 Da
• NCBI Official Full Name: glypican-3 isoform 4
• NCBI Official Synonym Full Names: glypican 3
• NCBI Official Symbol: GPC3
• NCBI Official Synonym Symbols: SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
• NCBI Protein Information: glypican-3; secreted glypican-3; glypican proteoglycan 3; intestinal protein OCI-5; heparan sulphate proteoglycan
• UniProt Protein Name: Glypican-3
• Protein Family: Glypican
• UniProt Gene Name: GPC3
• UniProt Synonym Gene Names: OCI5
• UniProt Entry Name: GPC3_HUMAN

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Uniprot Description

GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: lysosomal lumen; proteinaceous extracellular matrix; extracellular space; anchored to plasma membrane; Golgi lumen; integral to plasma membrane; plasma membrane

Molecular Function: heparan sulfate proteoglycan binding; protein binding

Biological Process: phototransduction, visible light; glycosaminoglycan metabolic process; negative regulation of peptidase activity; positive regulation of endocytosis; pathogenesis; osteoclast differentiation; embryonic hindlimb morphogenesis; bone mineralization; body morphogenesis; positive regulation of glucose import; glycosaminoglycan catabolic process; positive regulation of smoothened signaling pathway; retinoid metabolic process; positive regulation of BMP signaling pathway; negative regulation of growth; negative regulation of epithelial cell proliferation; anatomical structure morphogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; ureteric bud branching; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; positive regulation of protein catabolic process; anterior/posterior axis specification; lung development

Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1