Mouse anti-Human, Mouse Betaine-Homocysteine Methyltransferase Monoclonal Antibody | anti-BHMT antibody

Betaine-Homocysteine Methyltransferase (BHMT, Betaine-Homocysteine S-Methyltransferase)

Cat. Num. AAA603297

• Gene Names: BHMT; BHMT1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Betaine-Homocysteine Methyltransferase; Monoclonal Antibody; Betaine-Homocysteine Methyltransferase (BHMT; Betaine-Homocysteine S-Methyltransferase); Anti -Betaine-Homocysteine Methyltransferase (BHMT; anti-BHMT antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 7G21
• Specificity: Recognizes human BHMT at ~45kD. Species Crossreactivity: Mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-BHMT antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:500-1:1000
• Immunogen: Recombinant human BHMT (1-406aa) (E. coli)
• Hybridoma: Sp2/0 myeloma cells with spleen cells from BALB/c mice.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot of mouse liver using MBS603297.)

Related Product Information for anti-BHMT antibody

Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulfur amino acids, choline and betaine.

Product Categories/Family for anti-BHMT antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 157266337
• NCBI GeneID: 635
• NCBI Accession #: NP_001704.2
• NCBI GenBank Nucleotide #: NM_001713.2
• UniProt Accession #: Q93088; Q9UNI9; Q6EI07
• Molecular Weight: 44,998 Da
• NCBI Official Full Name: betaine--homocysteine S-methyltransferase 1
• NCBI Official Synonym Full Names: betaine--homocysteine S-methyltransferase
• NCBI Official Symbol: BHMT
• NCBI Official Synonym Symbols: BHMT1
• NCBI Protein Information: betaine--homocysteine S-methyltransferase 1; OTTHUMP00000135297
• UniProt Protein Name: Betaine--homocysteine S-methyltransferase 1
• Protein Family: Betaine--homocysteine S-methyltransferase
• UniProt Gene Name: BHMT
• UniProt Entry Name: BHMT1_HUMAN

NCBI Description

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]

Uniprot Description

BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.5

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: protein complex; cytoplasm; cytosol

Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding

Biological Process: S-methylmethionine cycle; protein amino acid methylation; betaine catabolic process; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; regulation of homocysteine metabolic process; betaine metabolic process