Chemiluminescence
(MBS619297(0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat BHMT Polyclonal Antibody | anti-BHMT antibody
BHMT (Betaine-homocysteine methyltransferase, BHMT)
Purified by affinity chromatography.
Purified by affinity chromatography.
Dilution: Peptide ELISA: 1:16,000
Western Blot: 0.03-0.1ug/ml ~45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293.
Immunohistochemistry: 2-4ug/ml In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells.
Other dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
(MBS619297(0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
(MBS619297(0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Testing Data
(HEK293 overexpressing BHMT and probed with MBS619297 (mock transfection in first lane), tested by Origene.)
Testing Data
(HEK293 overexpressing BHMT and probed with MBS619297 (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]
Uniprot Description
BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Protein type: Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.5
Chromosomal Location of Human Ortholog: 5q14.1
Cellular Component: protein complex; cytoplasm; cytosol
Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding
Biological Process: S-methylmethionine cycle; betaine catabolic process; protein amino acid methylation; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; betaine metabolic process; regulation of homocysteine metabolic process
Research Articles on BHMT
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Product Notes
The BHMT bhmt (Catalog #AAA619297) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The BHMT (Betaine-homocysteine methyltransferase, BHMT) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BHMT can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Peptide ELISA: 1:16,000 Western Blot: 0.03-0.1ug/ml ~45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Immunohistochemistry: 2-4ug/ml In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells. Other dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Researchers should empirically determine the suitability of the BHMT bhmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BHMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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