Western Blot (WB)
(Figure 1: Figure 1: Western blot analysis using BHMT mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY BHMT cDNA (2).)
Mouse anti-Human BHMT Monoclonal Antibody | anti-BHMT antibody
BHMT Antibody
Gene Names
BHMT; BHMT1; HEL-S-61p
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
BHMT; Monoclonal Antibody; BHMT Antibody; Betaine homocysteine methyltransferase; Betaine homocysteine S methyltransferase 1; Betaine homocysteine S methyltransferase; Betaine--homocysteine S-methyltransferase 1; BHMT1; BHMT1_HUMAN; EC 2.1.1.5; Epididymis secretory sperm binding protein Li 61p; HEL S 61p; anti-BHMT antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
BHMT antibody detects endogenous levels of total BHMT
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
406
Applicable Applications for anti-BHMT antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
WB: 1:500-1:2000
IHC: 1:200-1:1000
Immunogen
Purified recombinant fragment of human BHMT expressed in E. Coli
Subcellular Location
Cytoplasm.
Tissue Specificity
Found exclusively in liver and kidney.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Figure 1: Western blot analysis using BHMT mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY BHMT cDNA (2).)
Western Blot (WB)
(Figure 1: Figure 1: Western blot analysis using BHMT mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY BHMT cDNA (2).)
Related Product Information for anti-BHMT antibody
Description: BHMT: betaine-homocysteine methyltransferase. This protein is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
Function: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Subunit Structure: Homotetramer.
Function: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Subunit Structure: Homotetramer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 45 kDa
Predicted: 45 kDa
Predicted: 45 kDa
NCBI Official Full Name
betaine--homocysteine S-methyltransferase 1
NCBI Official Synonym Full Names
betaine--homocysteine S-methyltransferase
NCBI Official Symbol
BHMT
NCBI Official Synonym Symbols
BHMT1; HEL-S-61p
NCBI Protein Information
betaine--homocysteine S-methyltransferase 1
UniProt Protein Name
Betaine--homocysteine S-methyltransferase 1
Protein Family
UniProt Gene Name
BHMT
NCBI Description
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Research Articles on BHMT
Similar Products
Product Notes
The BHMT bhmt (Catalog #AAA9602431) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BHMT Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BHMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000. Researchers should empirically determine the suitability of the BHMT bhmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BHMT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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