Human Betaine Homocysteine Methyltransferase (BHMT) ELISA Kit | BHMT elisa kit

Human Betaine Homocysteine Methyltransferase (BHMT) ELISA Kit

Cat. Num. AAA2024260

• Gene Names: BHMT; BHMT1; HEL-S-61p
• Reactivity: Human
• Synonyms: Betaine Homocysteine Methyltransferase (BHMT); Human Betaine Homocysteine Methyltransferase (BHMT) ELISA Kit; Betaine-homocysteine S-methyltransferase; BHMT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Betaine Homocysteine Methyltransferase (BHMT).; No significant cross-reactivity or interference between Betaine Homocysteine Methyltransferase (BHMT) and analogues was observed.
• Sequence Length: 406

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Betaine Homocysteine Methyltransferase (BHMT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Betaine Homocysteine Methyltransferase (BHMT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BHMT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Betaine Homocysteine Methyltransferase (BHMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Betaine Homocysteine Methyltransferase (BHMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for BHMT elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 37222747
• NCBI GeneID: 635
• UniProt Accession #: Q93088; Q9UNI9
• Molecular Weight: 44,998 Da
• NCBI Official Full Name: betaine homocysteine methyltransferase, partial
• NCBI Official Synonym Full Names: betaine--homocysteine S-methyltransferase
• NCBI Official Symbol: BHMT
• NCBI Official Synonym Symbols: BHMT1; HEL-S-61p
• NCBI Protein Information: betaine--homocysteine S-methyltransferase 1
• UniProt Protein Name: Betaine--homocysteine S-methyltransferase 1
• Protein Family: Betaine--homocysteine S-methyltransferase
• UniProt Gene Name: BHMT
• UniProt Entry Name: BHMT1_HUMAN

NCBI Description

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protein type: EC 2.1.1.5; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - cysteine and methionine; Methyltransferase

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: protein complex; cytoplasm; cytosol

Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding

Biological Process: S-methylmethionine cycle; protein amino acid methylation; betaine catabolic process; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; betaine metabolic process; regulation of homocysteine metabolic process