Chicken Betaine--homocysteine S-methyltransferase 1 (BHMT) ELISA Kit | BHMT elisa kit

Chicken Betaine--homocysteine S-methyltransferase 1 (BHMT) ELISA Kit

Cat. Num. AAA7224784

• Gene Names: BHMT; BHMT1; HEL-S-61p
• Reactivity: Chicken
• Synonyms: Betaine--homocysteine S-methyltransferase 1 (BHMT); Chicken Betaine--homocysteine S-methyltransferase 1 (BHMT) ELISA Kit; BHMT elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of BHMT. No significant cross-reactivity or interference between BHMT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BHMT and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BHMT elisa kit

Intended Uses: This BHMT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken BHMT. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: BHMT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BHMT antibody and an BHMT-HRP conjugate. The assay sample and buffer are incubated together with BHMT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BHMT concentration since BHMT from samples and BHMT-HRP conjugate compete for the anti-BHMT antibody binding site. Since the number of sites is limited, as more sites are occupied by BHMT from the sample, fewer sites are left to bind BHMT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BHMT concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BHMT elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 157266337
• NCBI GeneID: 635
• NCBI Accession #: NP_001704.2
• NCBI GenBank Nucleotide #: NM_001713.2
• Molecular Weight: 44,998 Da
• NCBI Official Full Name: betaine--homocysteine S-methyltransferase 1
• NCBI Official Synonym Full Names: betaine--homocysteine S-methyltransferase
• NCBI Official Symbol: BHMT
• NCBI Official Synonym Symbols: BHMT1; HEL-S-61p
• NCBI Protein Information: betaine--homocysteine S-methyltransferase 1; epididymis secretory sperm binding protein Li 61p
• UniProt Protein Name: Betaine--homocysteine S-methyltransferase 1
• Protein Family: Betaine--homocysteine S-methyltransferase
• UniProt Gene Name: BHMT
• UniProt Entry Name: BHMT1_HUMAN

NCBI Description

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; EC 2.1.1.5; Methyltransferase; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: protein complex; cytoplasm; cytosol

Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding

Biological Process: S-methylmethionine cycle; betaine catabolic process; protein amino acid methylation; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; betaine metabolic process; regulation of homocysteine metabolic process