Testing Data
(Detection of cleaved IkBalpha in Jurkat cell lysates. Lane A. untreated cell lysate. Lane B. Jurkat cells treated with anti-Fas antibody for 30 mins.)
Mouse IkB alpha, Cleaved Monoclonal Antibody
IkB alpha, Cleaved
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G Affinity Chromatography.
Synonyms
IkB alpha; Cleaved; Monoclonal Antibody; Anti -IkB alpha; anti-IkB alpha; Cleaved antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4H110
Specificity
Recognizes human IkB alpha, Cleaved
Purity/Purification
Affinity Purified
Purified by Protein G Affinity Chromatography.
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Applicable Applications for anti-IkB alpha, Cleaved antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1ug/ml
Optimal dilution determined by the researcher.
Dilution: Western Blot: 1ug/ml
Optimal dilution determined by the researcher.
Immunogen
Synthetic peptide corresponding to the caspase-3 mediated cleavage site.
Positive Control
anti-Fas treated jurkat (30 min)
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Detection of cleaved IkBalpha in Jurkat cell lysates. Lane A. untreated cell lysate. Lane B. Jurkat cells treated with anti-Fas antibody for 30 mins.)
Testing Data
(Detection of cleaved IkBalpha in Jurkat cell lysates. Lane A. untreated cell lysate. Lane B. Jurkat cells treated with anti-Fas antibody for 30 mins.)
Related Product Information for anti-IkB alpha, Cleaved antibody
NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa) (7,8). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis.
Product Categories/Family for anti-IkB alpha, Cleaved antibody
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Product Notes
The IkB alpha, Cleaved (Catalog #AAA602080) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IkB alpha, Cleaved can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1ug/ml Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the IkB alpha, Cleaved for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IkB alpha, Cleaved, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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