Western Blot (WB)
(Western blot analysis of NF-kB p65-NLS in Jurkat cell lysate using MBS6010580 at 1ug/ml. goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)
Rabbit NFkB, p65, NLS Specific Polyclonal Antibody
NFkB, p65, NLS Specific (NF-kB, Nuclear Factor kappa B, RelA)
Reactivity
Bovine, Equine, Human, Monkey, Mouse
Applications
Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
NFkB; p65; NLS Specific; Polyclonal Antibody; NLS Specific (NF-kB; Nuclear Factor kappa B; RelA); Anti -NFkB; anti-NFkB; NLS Specific antibody
Host
Rabbit
Reactivity
Bovine, Equine, Human, Monkey, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NFkB, p65, NLS Specific. Species Crossreactivity: bovine, chimpanzee, gorilla, equine, monkey, mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Applicable Applications for anti-NFkB, p65, NLS Specific antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot and Immunocytochemistry.
Dilution: Western Blot: 1-3ug/ml
Immunocytochemistry: 5ug/ml
Dilution: Western Blot: 1-3ug/ml
Immunocytochemistry: 5ug/ml
Immunogen
A portion of the NF-kB p65 nuclear localization signal (DTDDRHRIEEKRKRKT).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of NF-kB p65-NLS in Jurkat cell lysate using MBS6010580 at 1ug/ml. goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)
Western Blot (WB)
(Western blot analysis of NF-kB p65-NLS in Jurkat cell lysate using MBS6010580 at 1ug/ml. goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)
Immunocytochemistry (ICC)
(Immunocytochemical analysis of NF-kB p65-NLS in formalin-fixed, paraffin-embedded human HeLa cells (top left) and LPS-stimulated HeLa cells (bottom left, right) using MBS6010580 at 5ug/ml.)
Immunocytochemistry (ICC)
(Immunocytochemical analysis of NF-kB p65-NLS in formalin-fixed, paraffin-embedded human HeLa cells (top left) and LPS-stimulated HeLa cells (bottom left, right) using MBS6010580 at 5ug/ml.)
Related Product Information for anti-NFkB, p65, NLS Specific antibody
P65 NLS is a transcription factor belonging to NF-kappa B family with the N-terminal Rel homology domain of the NF-kB family, a C-terminal transcription activation domain and a nuclear receptor interaction motif (LXXLL). p65 is a part of the nuclear localization signal (NLS) polypeptide of NF-kB (p50/p65) heterodimer. IkBa the most abundant IkB interacts with active NF-kB p65 homodimers and inactivates their transcriptional potential by trapping them in the cytoplasm and through stable complex formation. It is ubiquitously expressed in most of the tissues.
Product Categories/Family for anti-NFkB, p65, NLS Specific antibody
Similar Products
Product Notes
The NFkB, p65, NLS Specific (Catalog #AAA6010580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFkB, p65, NLS Specific (NF-kB, Nuclear Factor kappa B, RelA) reacts with Bovine, Equine, Human, Monkey, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NFkB, p65, NLS Specific can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in Western Blot and Immunocytochemistry. Dilution: Western Blot: 1-3ug/ml Immunocytochemistry: 5ug/ml. Researchers should empirically determine the suitability of the NFkB, p65, NLS Specific for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB, p65, NLS Specific, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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