Western Blot (WB)
(Western blot analysis of human GRAIL in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen and 4) rat spleen using MBS6009208 at 1ug/ml.)
Rabbit RNF128 Polyclonal Antibody
RNF128 (E3 Ubiquitin-protein Ligase RNF128, Gene Related to Anergy in Lymphocytes Protein, RING Finger Protein 128, GRAIL)
Reactivity
Canine, Equine, Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
RNF128; Polyclonal Antibody; RNF128 (E3 Ubiquitin-protein Ligase RNF128; Gene Related to Anergy in Lymphocytes Protein; RING Finger Protein 128; GRAIL); Anti -RNF128 (E3 Ubiquitin-protein Ligase RNF128; anti-RNF128 antibody
Host
Rabbit
Reactivity
Canine, Equine, Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RNF128. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Applicable Applications for anti-RNF128 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.5-2ug/ml
Immunohistochemistry (paraffin): 5-10ug/ml
Dilution: Western Blot: 0.5-2ug/ml
Immunohistochemistry (paraffin): 5-10ug/ml
Immunogen
Synthetic peptide corresponding to a (IFYSARRLRNARAQSRKQRQLKADAKK) portion of aa227-253 of human GRAIL, GenBank no. ref|NP_919445.1|.
Positive Control
Human, mouse and rat spleen lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of human GRAIL in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen and 4) rat spleen using MBS6009208 at 1ug/ml.)
Western Blot (WB)
(Western blot analysis of human GRAIL in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen and 4) rat spleen using MBS6009208 at 1ug/ml.)
Immunohistochemistry (IHC)
(IHC staining of normal human brain using MBS6009208 (GRAIL) at 5ug/ml.)
Immunohistochemistry (IHC)
(IHC staining of normal human brain using MBS6009208 (GRAIL) at 5ug/ml.)
Immunohistochemistry (IHC)
(IHC staining of normal human brain using control (rabbit Ig) at 5ug/ml.)
Immunohistochemistry (IHC)
(IHC staining of normal human brain using control (rabbit Ig) at 5ug/ml.)
Related Product Information for anti-RNF128 antibody
GRAIL is a type I transmembrane protein that localizes to the endocytic pathway and contains a PA (protease associated) domain and a RING-type zinc finger domain. It is a ubiquitin-protein isopeptide ligase (E3) necessary for the induction of CD4(+) T cell anergy in vivo and induced expression of this protein was observed in anergic CD4 (+) T cells, which suggested a role in the induction of anergic phenotype. It is differentially expressed in naturally occurring and peripherally induced CD25 (+) T regulatory cells and the expression is linked to the conversion of these cells to a regulatory phenotype. Expression of GRAIL in retrovirally transduced T cell hybridomas limits activation-induced IL-2 and IL-4 cytokine production. It also functions in the patterning of the dorsal ectoderm and sensitizes ectoderm to respond to neural-inducing signals.
Product Categories/Family for anti-RNF128 antibody
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Product Notes
The RNF128 (Catalog #AAA6009208) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF128 (E3 Ubiquitin-protein Ligase RNF128, Gene Related to Anergy in Lymphocytes Protein, RING Finger Protein 128, GRAIL) reacts with Canine, Equine, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RNF128 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 0.5-2ug/ml Immunohistochemistry (paraffin): 5-10ug/ml. Researchers should empirically determine the suitability of the RNF128 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF128, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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