Western Blot (WB)
(Western blot analysis of human RNF174 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using MBS6006274 at 0.5ug/ml.)
Rabbit MARCH4 Polyclonal Antibody
MARCH4 (E3 Ubiquitin-protein Ligase MARCH4, Membrane-associated RING Finger Protein 4, Membrane-associated RING-CH Protein IV, MARCH-IV, RING Finger Protein 174, KIAA1399, RNF174)
Reactivity
Equine, Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
MARCH4; Polyclonal Antibody; MARCH4 (E3 Ubiquitin-protein Ligase MARCH4; Membrane-associated RING Finger Protein 4; Membrane-associated RING-CH Protein IV; MARCH-IV; RING Finger Protein 174; KIAA1399; RNF174); Anti -MARCH4 (E3 Ubiquitin-protein Ligase MARCH4; anti-MARCH4 antibody
Host
Rabbit
Reactivity
Equine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MARCH4. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Applicable Applications for anti-MARCH4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.5-2ug/ml
Immunohistochemistry (paraffin): 10ug/ml
Dilution: Western Blot: 0.5-2ug/ml
Immunohistochemistry (paraffin): 10ug/ml
Immunogen
Synthetic peptide corresponding to aa300-350 of human RNF174.
Positive Control
Jurkat cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of human RNF174 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using MBS6006274 at 0.5ug/ml.)
Western Blot (WB)
(Western blot analysis of human RNF174 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using MBS6006274 at 0.5ug/ml.)
Immunohistochemistry (IHC)
(IHC analysis of human liver using MBS6006274 at 5ug/ml.)
Immunohistochemistry (IHC)
(IHC analysis of human liver using MBS6006274 at 5ug/ml.)
Related Product Information for anti-MARCH4 antibody
RNF174 (MARCH4) is a multi-pass membrane protein mainly found in the membrane of Golgi apparatus. It is a complex enzyme with a RING-CH-type zinc finger. It acts as a ubiquitin-protein ligase mainly involved in third step of ubiquitin conjugation. The RINGv-type zinc finger domain is required for E3 ligase activity in which the E3 ubiquitin-protein ligase mediates ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. It is mainly expressed in brain and placenta.
Product Categories/Family for anti-MARCH4 antibody
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Product Notes
The MARCH4 (Catalog #AAA6006274) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARCH4 (E3 Ubiquitin-protein Ligase MARCH4, Membrane-associated RING Finger Protein 4, Membrane-associated RING-CH Protein IV, MARCH-IV, RING Finger Protein 174, KIAA1399, RNF174) reacts with Equine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MARCH4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 0.5-2ug/ml Immunohistochemistry (paraffin): 10ug/ml. Researchers should empirically determine the suitability of the MARCH4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCH4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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