Rabbit anti-Human SLC5A12 Polyclonal Antibody | anti-SLC5A12 antibody

SLC5A12 Antibody (C-term)

Cat. Num. AAA9217008

• Gene Names: SLC5A12; SMCT2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
• Synonyms: SLC5A12; Polyclonal Antibody; SLC5A12 Antibody (C-term); Sodium-coupled monocarboxylate transporter 2; Electroneutral sodium monocarboxylate cotransporter; Low-affinity sodium-lactate cotransporter; Solute carrier family 5 member 12; SMCT2; anti-SLC5A12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SLC5A12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-605 amino acids from the C-terminal region of human SLC5A12.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 576-605

• Applicable Applications for anti-SLC5A12 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC), ELISA (EIA)
• Application Notes: WB~~1:2000; IHC-P~~1:50~100; FC~~1:10~50
• Function: Acts as an electroneutral and low-affinity sodium (Na(+))-dependent sodium-coupled solute transporter. Catalyzes the transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, nicotinate, propionate, butyrate and beta-D- hydroxybutyrate. May be responsible for the first step of reabsorption of monocarboxylates from the lumen of the proximal tubule of the kidney and the small intestine. May play also a role in monocarboxylates transport in the retina (By similarity). Mediates electroneutral uptake of lactate, with a stoichiometry of 2 Na(+) for each lactate (By similarity).
• Cellular Location: Apical cell membrane; Multi-pass membrane protein. Note=Detected at the brush border membrane of the kidney. Colocalizes with viementin in Mueller cells (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-SLC5A12 Antibody (C-term) at 1:2000 dilution + Y79 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(SLC5A12 antibody (C-term)(MBS9127008) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC5A12 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SLC5A12 Antibody (C-term)(MBS9127008) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SLC5A12 antibody

Normal blood lactate is maintained at about 1.5 mM, and little filtered lactate is excreted in urine. Reabsorption of lactate is mediated by the low-affinity Na(+)-coupled lactate transporter SLC5A12 in the initial part of the proximal tubule and by the high-affinity Na(+)-coupled lactate transporter SLC5A8 (MIM608044) in the distal proximal tubule (Gopal et al., 2007 [PubMed17692818]).

Product Categories/Family for anti-SLC5A12 antibody

Metabolism; Neuroscience; Signal Transduction

References

Gopal, E., et al. Biochim. Biophys. Acta 1768(11):2690-2697(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 157671931
• NCBI GeneID: 159963
• NCBI Accession #: NP_848593.2
• NCBI GenBank Nucleotide #: NM_178498.3
• UniProt Accession #: Q1EHB4; Q86UC7
• NCBI Official Full Name: sodium-coupled monocarboxylate transporter 2
• NCBI Official Synonym Full Names: solute carrier family 5 (sodium/monocarboxylate cotransporter), member 12
• NCBI Official Symbol: SLC5A12
• NCBI Official Synonym Symbols: SMCT2
• NCBI Protein Information: sodium-coupled monocarboxylate transporter 2
• UniProt Protein Name: Sodium-coupled monocarboxylate transporter 2
• Protein Family: Sodium-coupled monocarboxylate transporter
• UniProt Gene Name: SLC5A12
• UniProt Synonym Gene Names: SMCT2
• UniProt Entry Name: SC5AC_HUMAN

NCBI Description

Normal blood lactate is maintained at about 1.5 mM, and little filtered lactate is excreted in urine. Reabsorption of lactate is mediated by the low-affinity Na(+)-coupled lactate transporter SLC5A12 in the initial part of the proximal tubule and by the high-affinity Na(+)-coupled lactate transporter SLC5A8 (MIM 608044) in the distal proximal tubule (Gopal et al., 2007 [PubMed 17692818]).[supplied by OMIM, Dec 2008]

Uniprot Description

SLC5A12: Acts as an electroneutral and low-affinity sodium (Na(+))-dependent sodium-coupled solute transporter. Catalyzes the transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, nicotinate, propionate, butyrate and beta-D- hydroxybutyrate. May be responsible for the first step of reabsorption of monocarboxylates from the lumen of the proximal tubule of the kidney and the small intestine. May play also a role in monocarboxylates transport in the retina. Mediates electroneutral uptake of lactate, with a stoichiometry of 2 Na(+) for each lactate. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p14.2

Cellular Component: apical plasma membrane; integral to membrane

Molecular Function: lactate transmembrane transporter activity; symporter activity

Biological Process: sodium ion transport