Rabbit anti-Human RNF128 Polyclonal Antibody | anti-RNF128 antibody

RNF128, ID (RNF128, GRAIL, E3 ubiquitin-protein ligase RNF128, Gene related to anergy in lymphocytes protein, RING finger protein 128) (MaxLight 750)

Cat. Num. AAA6342564

• Gene Names: RNF128; GRAIL
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: RNF128; Polyclonal Antibody; ID (RNF128; GRAIL; E3 ubiquitin-protein ligase RNF128; Gene related to anergy in lymphocytes protein; RING finger protein 128) (MaxLight 750); RING finger protein 128; anti-RNF128 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RNF128.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-RNF128 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 115-143 in the central region of human RNF128, UniProt Accession #Q8TEB7. Species sequence homology: bovine
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB453 cell line lysates (35ug/lane) usingMBS644902. This demonstrates that MBS644902 detected the RNF128 protein (arrow).)

Related Product Information for anti-RNF128 antibody

The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Product Categories/Family for anti-RNF128 antibody

Antibodies; Ubiquitin Ligases

References

1. Su, L.L., et al. J. Immunol. 183(1):438-444(2009). 2. Lin, J.T., et al. J. Immunol. 182(10):5919-5928(2009). 3. Lineberry, N., et al. J. Biol. Chem. 283(42):28497-28505(2008). 4. Egawa, S., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 295 (1), G163-G169 (2008). 5. Kostianovsky, A.M., et al. J. Immunol. 178(10):6158-6163(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 37588871
• NCBI GeneID: 79589
• NCBI Accession #: NP_078815.3
• NCBI GenBank Nucleotide #: NM_024539.3
• UniProt Accession #: Q8TEB7; Q6PH80; Q6ZTJ8; Q96RF3; Q9H5E4; A0PJI4
• Molecular Weight: 44,617 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF128 isoform 2
• NCBI Official Synonym Full Names: ring finger protein 128, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF128
• NCBI Official Synonym Symbols: GRAIL
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF128
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF128
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF128
• UniProt Synonym Gene Names: GRAIL
• UniProt Entry Name: RN128_HUMAN