Rabbit RNF128 Polyclonal Antibody | anti-RNF128 antibody

RNF128 Polyclonal Antibody

Cat. Num. AAA3005621

• Gene Names: RNF128; GRAIL
• Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Zebrafish
• Applications: Western Blot, Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: RNF128; Polyclonal Antibody; RNF128 Polyclonal Antibody; E3 ubiquitin protein ligase RNF128; E3 ubiquitin-protein ligase RNF128; FLJ23516; Gene related to anergy in lymphocytes protein; GRAIL; ring finger protein 128; RN128_HUMAN; anti-RNF128 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Zebrafish
• Clonality: Polyclonal
• Specificity: RNF128 Polyclonal Antibody detects endogenous levels of RNF128 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-RNF128 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-2000; IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human RNF128:151-260/428
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Testing Data

(Primary: Anti- RNF128 at 1/1000 dilution)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Related Product Information for anti-RNF128 antibody

The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

NCBI and Uniprot Product Information

• NCBI GI #: 37588871
• NCBI GeneID: 79589
• NCBI Accession #: NP_078815.3
• NCBI GenBank Nucleotide #: NM_024539.3
• UniProt Accession #: Q8TEB7; Q6PH80; Q6ZTJ8; Q96RF3; Q9H5E4; A0PJI4
• Molecular Weight: 44,617 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF128 isoform 2
• NCBI Official Synonym Full Names: ring finger protein 128, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF128
• NCBI Official Synonym Symbols: GRAIL
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF128
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF128
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF128
• UniProt Synonym Gene Names: GRAIL
• UniProt Entry Name: RN128_HUMAN