Mouse anti-Human Nucleoli Monoclonal Antibody | anti-Npm1 antibody

Nucleoli

Cat. Num. AAA600586

• Gene Names: Npm1; B23; Npm; NO38
• Reactivity: Human
• Applications: Immunofluorescence
• Purity: Supernatant; Hybridoma supernatant, concentrated by ammonium sulfate.
• Synonyms: Nucleoli; Monoclonal Antibody; Anti -Nucleoli; anti-Npm1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q2233
• Specificity: Gives diffuse nucleolar staining pattern on all human cell types.
• Purity/Purification: Supernatant; Hybridoma supernatant, concentrated by ammonium sulfate.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-Npm1 antibody: Immunofluorescence (IF)
• Application Notes: Suitable for use in Indirect Immunofluorescence.; Dilution: Indirect immunofluorescence: 1:20-1:30. Use 30-100ul per well/slide.
• Immunogen: Human Raji cell nuclei.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(MBS600586 staining of nucleoli (green) in human neural stem cells, in vitro.)

Product Categories/Family for anti-Npm1 antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 2500582
• NCBI GeneID: 18148
• UniProt Accession #: Q61937
• Molecular Weight: 32,560 Da
• NCBI Official Full Name: Nucleophosmin
• NCBI Official Synonym Full Names: nucleophosmin 1
• NCBI Official Symbol: Npm1
• NCBI Official Synonym Symbols: B23; Npm; NO38
• NCBI Protein Information: nucleophosmin; numatrin; nucleolar protein NO38; nucleolar phosphoprotein B23
• UniProt Protein Name: Nucleophosmin
• Protein Family: Nucleolin
• UniProt Gene Name: Npm1
• UniProt Synonym Gene Names: NPM
• UniProt Entry Name: NPM_MOUSE

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; Nucleolus; Translation; Oncoprotein

Cellular Component: centrosome; granular component; small ribosomal subunit; focal adhesion; cell; spindle pole centrosome; cytosol; ribonucleoprotein complex; nucleoplasm; cytoskeleton; membrane; cytoplasm; nucleolus; nuclear speck; intracellular; nucleus; large ribosomal subunit

Molecular Function: protein homodimerization activity; protein kinase inhibitor activity; histone binding; RNA binding; unfolded protein binding; transcription coactivator activity; protein kinase binding; ribosomal large subunit binding; rRNA binding; NF-kappaB binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; enzyme binding; nucleic acid binding; DNA binding; Tat protein binding; protein heterodimerization activity; ribosomal small subunit binding

Biological Process: positive regulation of catalytic activity; positive regulation of DNA metabolic process; regulation of centriole replication; positive regulation of translation; regulation of cell cycle; centrosome cycle; nucleocytoplasmic transport; ribosomal large subunit biogenesis and assembly; ribosomal large subunit export from nucleus; regulation of DNA damage response, signal transduction by p53 class mediator; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; protein localization; regulation of neuron apoptosis; positive regulation of cell proliferation; response to stress; cell growth; protein homooligomerization; ribosomal small subunit export from nucleus; protein destabilization; cell aging; DNA repair; positive regulation of cellular biosynthetic process; protein oligomerization; nucleosome assembly; ribosomal small subunit biogenesis and assembly; rRNA export from nucleus; regulation of endodeoxyribonuclease activity; negative regulation of nuclear mRNA splicing, via spliceosome; positive regulation of protein kinase activity; cell volume homeostasis; positive regulation of DNA replication; negative regulation of apoptosis