Rat anti-Mouse MBL-C Monoclonal Antibody | anti-MBL-C antibody

MBL-C, Mouse, mAb 14D12

Cat. Num. AAA584188

• Gene Names: Mbl2; MBL; L-MBP; MBL-C; MBP-C
• Reactivity: Mouse
• Applications: Immunofluorescence, Western Blot
• Synonyms: MBL-C; Monoclonal Antibody; Mouse; mAb 14D12; MBL; L-MB; MBP-C; Mbl2; anti-MBL-C antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 244

• Applicable Applications for anti-MBL-C antibody: F, Immunoassay (IA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Application Notes: F: Cryostat sections (7um) of mouse tissue were acetone fixed and stained o/n at 4 degree C in TBS/2% goat serum. (Ref.4); Application Use: For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. It is recommended that solutions with a calcium concentration of 1 mM are used (14D12 is a calcium-dependent antibody).
• Immunogen: Purified MBL-C
• Positive Control: Mouse Serum and kidney tissue
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-MBL-C antibody

Product Description: Mannose binding lectin (MBL), also called mannose- or mannan-binding protein (MBP), is a member of the group of collectins. MBL is an important pattern-recognition receptor in the innate immune system. The protein mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. Binding of MBL leads to the activation of MBL-associated serine proteases (MASP's). Activated MASP-2 cleaves C4 and C2 in a similar way as C1s do for the classical pathway (CP) leading to the formation of C4b2a, cleavage of the classical pathway convertase C3, and eventually complement activation up to the formation of the membrane attack complex. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways.
MBL is predominantly synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C.
The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. The monoclonal antibody 14D12 is a calcium-dependent antibody.
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References

1. Liu, H et al; Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. Scand J Immunol 2001, 53: 489 2. Phaneuf, L et al; Binding of mouse mannan-binding lectin to different bacterial pathogens of mice. Vet Immunol Immunopathol 2007, 118: 129
3. Held, K et al; Increased susceptibility of complement factor B/C2 double knockout mice and mannan binding lectin knockout mice to systemic infection with Candida albicans. Mol Immunol 2008, 45: 3934
4. Matthijsen, R et al; Macrophage-specific expression of mannose-binding lectin controls atherosclerosis in low-density lipoprotein receptor deficient mice. Circulation 2009, 119: 2188
5. Pumidonming, W et al, Binding to complement factors and activation of the alternative pathway by Acanthamoeba. Immunobiol. 2010, 216: 225

NCBI and Uniprot Product Information

• NCBI GI #: 6754656
• NCBI GeneID: 17195
• NCBI Accession #: NP_034906.1
• NCBI GenBank Nucleotide #: NM_010776.1
• Molecular Weight: 25,957 Da
• NCBI Official Full Name: mannose-binding protein C
• NCBI Official Synonym Full Names: mannose-binding lectin (protein C) 2
• NCBI Official Symbol: Mbl2
• NCBI Official Synonym Symbols: MBL; L-MBP; MBL-C; MBP-C
• NCBI Protein Information: mannose-binding protein C; RARF/P28A; mannan-binding protein; mannose binding lectin (C); RA-reactive factor P28A subunit; mannose binding lectin, liver (C)
• UniProt Protein Name: Mannose-binding protein C
• UniProt Gene Name: Mbl2
• UniProt Synonym Gene Names: MBP-C; RARF/P28A
• UniProt Entry Name: MBL2_MOUSE

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; collagen; extracellular region

Molecular Function: mannose binding; protease binding; protein self-association; galactose binding; calcium ion binding; carbohydrate binding; calcium-dependent protein binding; receptor binding

Biological Process: defense response to Gram-positive bacterium; immune system process; killing by host of symbiont cells; positive regulation of phagocytosis; innate immune response; complement activation, lectin pathway; negative regulation of viral reproduction; complement activation, classical pathway