Porcine VEGFR1/Flt1 ELISA Kit | VEGFR1/Flt1 elisa kit

Porcine VEGFR1/Flt1 (Vascuoar Endothelial Growth Factor Receptor 1) ELISA Kit

Cat. Num. AAA2515376

• Gene Names: FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
• Reactivity: Porcine
• Synonyms: VEGFR1/Flt1; Porcine VEGFR1/Flt1 (Vascuoar Endothelial Growth Factor Receptor 1) ELISA Kit; VEGFR1/Flt1 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Porcine VEGFR1/Flt1. No significant cross-reactivity or interference between Porcine VEGFR1/Flt1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Porcine VEGFR1/Flt1 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 541

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGFR1/Flt1 elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VEGFR1/Flt1 in Porcine serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine VEGFR1/Flt1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Porcine VEGFR1/Flt1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine VEGFR1/Flt1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine VEGFR1/Flt1. You can calculate the concentration of Porcine VEGFR1/Flt1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 229892302
• NCBI GeneID: 2321
• NCBI Accession #: NP_001153503.1
• NCBI GenBank Nucleotide #: NM_001160031.1
• UniProt Accession #: P17948; O60722; A3E342; A3E344; A8KA71; B0LPF1; B2BF46; B2BF47; B2BF48; B3FR89; B5A923; F5H5L6
• Molecular Weight: 41,175 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 1 isoform 4
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 1
• NCBI Official Symbol: FLT1
• NCBI Official Synonym Symbols: FLT; FLT-1; VEGFR1; VEGFR-1
• NCBI Protein Information: vascular endothelial growth factor receptor 1; fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• UniProt Gene Name: FLT1
• UniProt Synonym Gene Names: FLT; FRT; VEGFR1; VEGFR-1; FLT-1; FLT
• UniProt Entry Name: VGFR1_HUMAN

NCBI Description

This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009]

Uniprot Description

VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; EC 2.7.10.1; Kinase, protein; TK group; VEGFR family

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: extracellular space; focal adhesion; integral to plasma membrane; plasma membrane; endosome; receptor complex

Molecular Function: identical protein binding; vascular endothelial growth factor receptor activity; protein binding; growth factor binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; patterning of blood vessels; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; blood vessel morphogenesis; embryonic morphogenesis; sprouting angiogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration