Mouse anti-Human Melanoma Associated Antigen Monoclonal Antibody

Mouse anti Melanoma Associated Antigen

Cat. Num. AAA570200

• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry
• Synonyms: Melanoma Associated Antigen; Monoclonal Antibody; Mouse anti Melanoma Associated Antigen; anti-Melanoma Associated Antigen antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: NKI-C3
• Specificity: NKI/C-3 is a MAb which is directed anti a predominantly intracellular protein of Human melanoma cells. The antigen is abundantly present in malignant melanomas, nevocellular nevi, and neuroendocrine tumors, whereas it has not been detectable in most other tissues. The antigen recognized by NKI/C-3 is a glycoprotein which appears as a broad band in SDS-PAGE. The antigen was shown to consist of a single protein backbone to which two or three.V-linked glycans were added cotranslationally. Extensive further heterogeneity was geneRated in the Golgi compartment and was shown to be dependent on the presence of complex type sugars. Although the antigen is associated with melanomas, it was not codistributed with the tyrosinase activity associated with melanogenesis. The antigen did show codistribution with Cathepsin D, which is a marker for lysosomal functions. NKI/C3 originally was described as a marker for melanoma. Recently, it resurfaced as a marker to sepaRate cellular neurothekeoma from other dermal tumors in the differential.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-Melanoma Associated Antigen antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin)
• Application Notes: NKI-C3 is useful for ELISA, immunofluorescence and immuohistochemistry on frozen and paraffin embedded sections. Optimal antibody dilution should be determined by titRation.
• Source Note: NKI-C3 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Balb/c mice, immunized with a predominantly intracellular protein of Human melanoma cells.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Related Product Information for anti-Melanoma Associated Antigen antibody

The mammalian members of the MAGE (melanoma-associated antigen) gene family were originally described as completely silent in normal adult tissues, with the exception of male germ cells and, for some of them, placenta. By contrast, these genes were expressed in various kinds of tumors. MAGE-like genes have also been identified in non-mammalian species, like the zebrafish or Drosophila melanogaster. Although no MAGE homologous sequences have been identified in Caenorhabditis elegans, Saccharomyces cerevisiae or Schizosaccharomyces pombe, MAGE sequences have been found in several vegetal species, including Arabidopsis thaliana. The only region of homology shared by all of the members of the family is a stretch of about 200 amino acids which has been named the MAGE conserved domain. The MAGE conserved domain is usually loCated close to the C-terminal, although it can also be found in a more central position in some proteins. The MAGE conserved domain is generally present as a single copy but it is dupliCated in some proteins. It has been proposed that the MAGE conserved domain of MAGE-D proteins might interact with p75 neurotrophin or related receptors. The function of this protein is not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. The Melanoma-associated antigen is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, leukemias and lymphomas.

Product Categories/Family for anti-Melanoma Associated Antigen antibody

Tumour marker

References

C. Vennegoor et al., Int. J. Cancer 35: 287-295, 1985.A.A. Palmer et al., Pathology 17: 335-339, 1985. 3.E.C. Hagen et al., Histopathology 10: 689-700, 1986.

NCBI and Uniprot Product Information

• NCBI GI #: 3176846
• NCBI Official Full Name: melanoma-associated antigen

Uniprot Description

MAGE-C1: 2 isoforms of the human protein are produced by alternative splicing

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq26

Molecular Function: protein binding