Testing Data
(Gel: 10%SDS-PAGE Lysate: 40ug Hela cell Primary antibody: 1/1250 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)
Rabbit anti-Human NOL9 Polyclonal Antibody | anti-NOL9 antibody
NOL9 Antibody
Gene Names
NOL9; Grc3; NET6
Reactivity
Human
Applications
Western Blot
Purity
Antigen affinity purification.
Synonyms
NOL9; Polyclonal Antibody; NOL9 Antibody; Grc3; NET6; anti-NOL9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total NOL9 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
2.5 mg/ml (varies by lot)
Sequence Length
702
Applicable Applications for anti-NOL9 antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500-1:2000
Immunogen Type
Recombinant Protein
Immunogen Description
Fusion protein corresponding to residues near the C terminal of human nucleolar protein 9
Target Name
NOL9
Preparation and Storage
Store at -20 degree C
Testing Data
(Gel: 10%SDS-PAGE Lysate: 40ug Hela cell Primary antibody: 1/1250 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)
Testing Data
(Gel: 10%SDS-PAGE Lysate: 40ug Hela cell Primary antibody: 1/1250 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)
Related Product Information for anti-NOL9 antibody
Nucleolar protein 9 is a protein that in humans is encoded by the NOL9 gene. Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA.
Product Categories/Family for anti-NOL9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,323 Da
NCBI Official Full Name
polynucleotide 5'-hydroxyl-kinase NOL9
NCBI Official Synonym Full Names
nucleolar protein 9
NCBI Official Symbol
NOL9
NCBI Official Synonym Symbols
Grc3; NET6
NCBI Protein Information
polynucleotide 5'-hydroxyl-kinase NOL9
UniProt Protein Name
Polynucleotide 5'-hydroxyl-kinase NOL9
Protein Family
UniProt Gene Name
NOL9
UniProt Entry Name
NOL9_HUMAN
Uniprot Description
NOL9: Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA. Belongs to the Clp1 family. NOL9/GRC3 subfamily.
Protein type: Nucleolus; EC 2.7.1.-
Chromosomal Location of Human Ortholog: 1p36.31
Cellular Component: membrane; nucleolus
Molecular Function: RNA binding; polynucleotide kinase activity; ATP binding
Biological Process: cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); maturation of 5.8S rRNA; phosphorylation
Research Articles on NOL9
Similar Products
Product Notes
The NOL9 nol9 (Catalog #AAA9409595) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOL9 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOL9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500-1:2000. Researchers should empirically determine the suitability of the NOL9 nol9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOL9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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