Immunohistochemistry (IHC)
(Anti-Melanoma-Associated Antigen antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Mouse anti-Human Melanoma-Associated Antigen Monoclonal Antibody | anti-IgG1 antibody
Anti-Melanoma-Associated Antigen Antibody (clone NKI-C3) IHC-plus
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA
Purity
Purified
Synonyms
Melanoma-Associated Antigen; Monoclonal Antibody; Anti-Melanoma-Associated Antigen Antibody (clone NKI-C3) IHC-plus; Mouse Monoclonal [clone NKI-C3] (IgG1) to Human Melanoma-Associated Antigen; Human Melanoma-Associated Antigen; anti-IgG1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
NKI-C3
Specificity
Used for by staining of lysosomes/melanosomes. Directed against a predominantly intracellular protein of human melanoma cells. The antigen is abundantly present in malignant melanomas, nevocellular nevi, and neuroendocrine tumors, whereas it has not been detectable in most other tissues. The antigen recognized is a glycoprotein which appears as a broad band in SDS-PAGE. The antigen was shown to consist of a single protein backbone to which two or three V-linked glycans were added cotranslationally. Extensive further heterogeneity was generated in the Colgi compartmen and was shown to be dependent on the presence of complex type sugars. Although the antigen is associated with melanomas, it was not codistributed with the tyrosinase activity associated with melanogenesis. The antigen did show codistribution with cathepsin D, which is a marker for lysosomal functions.This antibody was originally described as a marker for melanoma. Recently, it resurfaced as a marker to separate cellular neurothekeoma from other dermal tumors in the differential.
Purity/Purification
Purified
Form/Format
PBS containing 0.09% sodium azide.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-IgG1 antibody
Immunohistochemistry (IHC) Formalin/Paraffin, Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
IHC-P (10 ug/ml)
Immunogen
Melanoma-associated antigen
Immunogen Description
Melanoma-associated antigen
Target Species
Human
Preparation and Storage
Short term 4 degree C
Long term aliquot and store at -20 degree C
Avoid freeze thaw cycles. Store undiluted.
Long term aliquot and store at -20 degree C
Avoid freeze thaw cycles. Store undiluted.
Immunohistochemistry (IHC)
(Anti-Melanoma-Associated Antigen antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-Melanoma-Associated Antigen antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
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Product Notes
The IgG1 (Catalog #AAA249253) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Melanoma-Associated Antigen Antibody (clone NKI-C3) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Melanoma-Associated Antigen can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin/Paraffin, Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA). IHC-P (10 ug/ml). Researchers should empirically determine the suitability of the IgG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Melanoma-Associated Antigen, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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