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Western Blot (WB) (Sample Data: Representative western blot from 293 cell lysate using ERK2 antibody (1:2500).)

Rabbit ERK2 Polyclonal Antibody | anti-ERK2 antibody

Anti-ERK2

Reactivity
ERK2 from human, rat, bovine, mouse, rabbit
ERK2 from other species may also be detectable.
Applications
Western Blot
Purity
Affinity purified by ERK2 c-terminal peptide on agarose from rabbit serum
Synonyms
ERK2; Polyclonal Antibody; Anti-ERK2; anti-ERK2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
ERK2 from human, rat, bovine, mouse, rabbit
ERK2 from other species may also be detectable.
Clonality
Polyclonal
Specificity
Anti-ERK2 antibody recognizes the ERK2 protein.
Purity/Purification
Affinity purified by ERK2 c-terminal peptide on agarose from rabbit serum
Form/Format
0.1M TRIS pH8.0, 0.1M glycine
Applicable Applications for anti-ERK2 antibody
Western Blot (WB)
Application Notes
WB: 20311:22810
Immunogen
Human ERK2 c-terminal peptide-KLH conjugates
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Western Blot (WB)

(Sample Data: Representative western blot from 293 cell lysate using ERK2 antibody (1:2500).)

Western Blot (WB) (Sample Data: Representative western blot from 293 cell lysate using ERK2 antibody (1:2500).)
Related Product Information for anti-ERK2 antibody
ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).
References
1. Boulton, TG. et al: Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase. Biochemistry. 1991 Jan 8;30(1):278-86.

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Product Notes

The ERK2 (Catalog #AAA516081) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ERK2 reacts with ERK2 from human, rat, bovine, mouse, rabbit ERK2 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's ERK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 20311:22810. Researchers should empirically determine the suitability of the ERK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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