Western Blot (WB)
(Sample Data: Western blots from NIH-3T3 cells using ERK1/2 antibody (lanes 1 and 2) and anti-phospho-ERK1/2 (Tyr204) antibody (lanes 3 and 4).)
Rabbit ERK1/2 Polyclonal Antibody | anti-phospho-ERK1/2 antibody
Anti-phospho-ERK1/2 (Tyr204)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity chromatography
Synonyms
ERK1/2; Polyclonal Antibody; Anti-phospho-ERK1/2 (Tyr204); anti-phospho-ERK1/2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the ERK1/2 protein phosphorylated at tyrosine 204
Purity/Purification
Affinity chromatography
Form/Format
PBS (pH7.4) 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-phospho-ERK1/2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr204
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blots from NIH-3T3 cells using ERK1/2 antibody (lanes 1 and 2) and anti-phospho-ERK1/2 (Tyr204) antibody (lanes 3 and 4).)
Western Blot (WB)
(Sample Data: Western blots from NIH-3T3 cells using ERK1/2 antibody (lanes 1 and 2) and anti-phospho-ERK1/2 (Tyr204) antibody (lanes 3 and 4).)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-ERK1/2 (Tyr204) antibody.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-ERK1/2 (Tyr204) antibody.)
Related Product Information for anti-phospho-ERK1/2 antibody
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is a serine threonine kinase. It plays an integral role of cellular signaling during mitogenesis and differentiation of mitotic cells. ERK is presumed to have a key role in learning and memory (1,2,3). The activity of this kinase is regulated by phosphorylation at Thr202 and Tyr204 (4). Activated ERK1/2 translocates into the nucleus where it phosphorylates various transcription factors (e.g Elk-1, c-Myc, c-Jun, c-Fos and C/EBP beta).
Product Categories/Family for anti-phospho-ERK1/2 antibody
References
1. Adams, J P. et al: Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 2002 42:135-163.
2. Johnson, G L. et al: Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 202 298:1911-1912.
3. Tanoue, T J. et al: Molecular recognitions in the MAP kinase cascades. Cellular Signaling 2003 15:455-462.
4. Ahn, N G.: The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 1993 127-128:201-209.
2. Johnson, G L. et al: Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 202 298:1911-1912.
3. Tanoue, T J. et al: Molecular recognitions in the MAP kinase cascades. Cellular Signaling 2003 15:455-462.
4. Ahn, N G.: The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 1993 127-128:201-209.
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Product Notes
The phospho-ERK1/2 (Catalog #AAA516087) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-ERK1/2 (Tyr204) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ERK1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the phospho-ERK1/2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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