Mouse anti-Rat, mouse NMDA NR1 Subunit Monoclonal Antibody | anti-GRIN1 antibody

Anti-NMDA Receptor, NR1 Subunit

Cat. Num. AAA500009

• Gene Names: Grin1; NR1; GluN1; NMDAR1
• Reactivity: Rat, mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Culture supernatant
• Synonyms: NMDA NR1 Subunit; Monoclonal Antibody; Anti-NMDA Receptor; NR1 Subunit; anti-GRIN1 antibody

• Host: Mouse
• Reactivity: Rat, mouse
• Clonality: Monoclonal
• Clone Number: R1JHL
• Specificity: Specific for the ~120k NR1 subunit of the NMDA receptor.
• Purity/Purification: Culture supernatant
• Form/Format: 15 ug antibody vial; lyophilized from culture supernatant. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody should b
• Sequence Length: 959

• Applicable Applications for anti-GRIN1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Quality Control: Western blots performed on each lot; WB: 1:1000; IHC: 1 ug per ml; IP: 3 ug per 200 ug lysate
• Antigen: Fusion protein containing amino acids 1-564 of the NR1 subunit of rat NMDA receptor.
• Immunogen Information: Fusion protein containing amino acids 1-564 of the NR1 subunit
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues
• Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1-subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-GRIN1 antibody

Mouse monoclonal antibody

References

• Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724
• Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neurosci 2:331-338.

NCBI and Uniprot Product Information

• NCBI GI #: 396578144
• NCBI GeneID: 24408
• NCBI Accession #: NP_001257531.1
• NCBI GenBank Nucleotide #: NM_001270602.1
• UniProt Accession #: P35439; Q62646
• Molecular Weight: 120
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 1 isoform 1b
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• NCBI Official Symbol: Grin1
• NCBI Official Synonym Symbols: NR1; GluN1; NMDAR1
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 1; NMD-R1; neurotransmitter receptor; NMDA R1 receptor C1 cassette; N-methyl-D-aspartate glutamate receptor; glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 1
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin1
• UniProt Synonym Gene Names: Nmdar1; GluN1; NMD-R1
• UniProt Entry Name: NMDZ1_RAT

NCBI Description

subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006]

Uniprot Description

Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. Ref.18

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked

By similarity. Found in a complex with GRIN2A or GRIN2B, GRIN3A and PPP2CB. Found in a complex with GRIN2A or GRIN2B and GRIN3B. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes

By similarity. Interacts with DLG4 and MPDZ. Interacts with LRFN1 and LRFN2. Interacts with MYZAP. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.17

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Cell junction › synapse › postsynaptic cell membrane

By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density

By similarity. Note: Enriched in postsynaptic plasma membrane and postsynaptic densities

By similarity.

Post-translational modification: NMDA is probably regulated by C-terminal phosphorylation of an isoform ofNR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. Ref.7 Ref.12

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. [View classification]