Western Blot (WB)
(Western Blot using anti-EpCAM antibody HEA125 (MBS488693). human breast carcinoma(A) and human colorectal carcinoma(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of HEA125 (MBS488692) at 1ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Rabbit anti-Human EpCAM Monoclonal Antibody | anti-EpCAM antibody
Anti-EpCAM [HEA125]
Western Blot (WB)
(Western Blot using anti-EpCAM antibody HEA125 (MBS488693). human breast carcinoma(A) and human colorectal carcinoma(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of HEA125 (MBS488692) at 1ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-EpCAM antibody HEA125 (MBS488693). human breast carcinoma(A) and human colorectal carcinoma(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of HEA125 (MBS488692) at 1ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of Caco-2 cells using anti-EpCAM (MBS488693) HEA125 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric mouse IgG version of HEA125 (MBS488692) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488526, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (MBS488140) followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of Caco-2 cells using anti-EpCAM (MBS488693) HEA125 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric mouse IgG version of HEA125 (MBS488692) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488526, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (MBS488140) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-EpCAM antibody HEA125 (MBS488693). Caco-2 cells were stained with anti-unknown IgG antibody (MBS488140; isotype control, black line) or the mouse IgG-chimeric version of HEA125 (MBS488692, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-EpCAM antibody HEA125 (MBS488693). Caco-2 cells were stained with anti-unknown IgG antibody (MBS488140; isotype control, black line) or the mouse IgG-chimeric version of HEA125 (MBS488692, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Western Blot (WB)
(Western Blot using anti-EpCAM antibody HEA125 (MBS488693). human breast carcinoma(A) and human colorectal carcinoma(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of HEA125 (MBS488692) at 1ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-EpCAM antibody HEA125 (MBS488693). human breast carcinoma(A) and human colorectal carcinoma(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of HEA125 (MBS488692) at 1ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-EpCAM antibody HEA125 (MBS488693). Caco-2 cells were stained with anti-unknown specificity antibody (MBS488140; isotype control, black line) or the mouse IgG1 version of HEA125 (MBS488692, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-EpCAM antibody HEA125 (MBS488693). Caco-2 cells were stained with anti-unknown specificity antibody (MBS488140; isotype control, black line) or the mouse IgG1 version of HEA125 (MBS488692, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
This antibody was raised by immunising BALB/c mice with the human colon carcinoma cell line HT-29.
Note on publication: Describes the original generation of this antibody, and its characterisation.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Uniprot Description
EPCAM: a single-pass type I membrane protein of the EPCAM family. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas including pancreatic carcinoma. May be a marker of cancer stem cells.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2p21
Cellular Component: tight junction; cell surface; basolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; lateral plasma membrane
Molecular Function: protein binding; protein complex binding
Biological Process: ureteric bud development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation
Disease: Diarrhea 5, With Tufting Enteropathy, Congenital; Colorectal Cancer, Hereditary Nonpolyposis, Type 8
Research Articles on EpCAM
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Product Notes
The EpCAM epcam (Catalog #AAA488693) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EpCAM [HEA125] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EpCAM can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Radioimmunoassay (RIA) (RIA). This antibody has applications in the detection of normal and neoplastic epithelial cells, and distinguishing carcinomas from non-epithelial neoplasms (Momburg et al, 1987). This antibody has been used in radioimmunoassays on live cells and immunoenzymatic staining assays on glutaraldehyde-fixed cells, as well as in immunoperoxidase staining of frozen and formalin-fixed paraffin tissue sections (Moldenhauer et al, 1987; Momburg et al, 1987). These revealed it to react with most normal human epithelial cells, but not non-epithelial tissue, and to intensely react with human carcinomas and metastatic lesions, but not lymphoma, melanoma, sarcoma or neuroblastoma cell lines (Moldenhauer et al, 1987; Momburg et al, 1987). Squamous cell carcinomas stain less strongly than adenocarcinomas, and keratinizing areas of tumor masses remain negative (Momburg et al, 1987). This antibody has also been used in immunoprecipitation analysis to characterise human EpCAM (Moldenhauer et al, 1987), and in immunohistochemical analysis of EpCAM expression in formalin-fixed, paraffin-embedded liver tissue sections (Igarashi et al, 2013; Wang et al, 2018) and frozen liver tissue sections (Crosby et al, 1998). Additionally, it has been used in the immunocytochemical detection of EpCAM expression in mononuclear cell preparations obtained from human peripheral blood or lamina propria (Qiao et al, 1996). Researchers should empirically determine the suitability of the EpCAM epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EpCAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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