Human C Reactive Protein (CRP) ELISA Kit | CRP elisa kit

Human C Reactive Protein (CRP) High Sensitive ELISA Kit

Cat. Num. AAA2708035

• Gene Names: CRP; PTX1
• Reactivity: Human
• Applications: ELISA
• Synonyms: C Reactive Protein (CRP); Human C Reactive Protein (CRP) High Sensitive ELISA Kit; C Reactive Protein; C-RP; PTX1; Pentraxin-Related; Pentraxin 1; CRP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CRP. No significant cross-reactivity or interference between CRP and analogues was observed.
• Sequence Length: 224

• Applicable Applications for CRP elisa kit: ELISA (EIA)
• Samples: Serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4,000pg/mL
• Sensitivity: < 24.3pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CRP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CRP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CRP in human serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CRP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55770842
• NCBI GeneID: 1401
• NCBI Accession #: NP_000558.2
• NCBI GenBank Nucleotide #: NP_000558.2
• UniProt Accession #: P02741
• NCBI Official Full Name: C-reactive protein isoform 1
• NCBI Official Synonym Full Names: C-reactive protein
• NCBI Official Symbol: CRP
• NCBI Official Synonym Symbols: PTX1
• NCBI Protein Information: C-reactive protein
• UniProt Protein Name: C-reactive protein
• Protein Family: Cryparin
• UniProt Gene Name: CRP
• UniProt Synonym Gene Names: PTX1
• UniProt Entry Name: CRP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

Uniprot Description

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: extracellular space; growth cone; extracellular region; filopodium

Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; cholesterol binding; low-density lipoprotein binding; virion binding; calcium ion binding

Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; negative regulation of vasodilation; wound healing; positive regulation of superoxide release; response to lead ion; response to hypoxia; acute-phase response; opsonization; inflammatory response; complement activation, classical pathway; aging