Tim-4 recombinant antibody

Anti-Tim-4 [5G3]

Cat. Num. AAA488480

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
• Purity: Protein A Affinity Purified
• Synonyms: Tim-4; Monoclonal Recombinant Antibody; Anti-Tim-4 [5G3]; TIMD4; SMUCKLER; TIM4; Spleen; mucin-containing; knockout of lymphotoxin protein; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIM-4; TIMD-4; Tim-4 recombinant antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG1 kappa
• Clone Number: 5G3
• Specificity: This antibody is specific for murine TIM-4, which unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen presenting cells such as lymphoid and myeloid DC's and macrophages. TIM-4 has been shown to interact with TIM-1 thereby suggesting a role of TIM-4 in the balance of Th1/Th2 regulation. Additionally TIM-4 has been shown to be a receptor for phosphatidylserine and may play a role in engulfment of apoptotic cells.
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 343

• Applicable Applications for Tim-4 recombinant antibody: Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Blocking (BL), Therapeutic
• Antibody Type: Recombinant Antibody
• Antibody Species: Rat
• Original Species Name: Rat
• Immunogen: This antibody was raised by immunising female Lewis rats (Harlan Sprague-Dawley) with Tim-4:Ig fusion protein in complete Freund's adjuvant.
• Clone Note: This antibody (Clone 5G3), together with two others (clones 3A1 and 3H11), was originally produced from the subcloned hybridomas; all of these 3 produced mAbs bound specifically to murine TIM-4 and not to any other TIM molecule, as demonstrated by both FACS and ELISA analyses (Rodriguez-Manzanet et al, 2008). This antibody has been used in various FACS analyses, for example, to show that TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells (Kobayashi et al, 2007), to underscore the requirement of IL-10 from marginal zone precursor B cell subset for costimulatory blockade-induced transplantation tolerance (Lal et al, 2015), and to uncover a mechanism by which the body adapts to fluctuations in erythrocyte integrity (Theurl et al, 2016). This antibody has also been used in immunohistochemistry to discover a new tissue macrophage subset in the thymus that is dependent on transcription factor NR4A1 for development (Tacke et al, 2015), as well as in immunofluorescence to elucidate the mechanisms that control the heterogeneity of tissue macrophages (A-Gonzalez et al, 2013). In addition, the anti-tumour effects of anti-TIM-4 antibodies have been demonstrated in various murine melanoma and colon adenocarcinoma models (Baghdadi et al, Cancer Immunol. Immunother. 2013; Baghdadi et al, Immunity. 2013).
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed mouse splenocytes with anti-Tim-4 antibody 5G3 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 5G3 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)

Related Product Information for Tim-4 recombinant antibody

Recombinant monoclonal antibody to Tim-4. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma 5G3.

Product Categories/Family for Tim-4 recombinant antibody

Immunology; Costimulation; Cell Signalling; Oncology; Immuno-oncology; Immune-checkpoint; T Cells

References

Rodriguez-Manzanet Roselynn et al. Tim-4 expressed on antigen-presenting cells induces T cell expansion and survival. J Immunol. 2008 Apr 1;180(7):4706-13. PMID:18354194
Original Published Journal: J Immunol. 2008 Apr 1;180(7):4706-13.
Publication Note: Describe the original generation of this antibody and its subsequent use in FACS and Western Blots for insights into Tim-4 as a costimulatory molecule that promotes T cell expansion and survival by crosslinking Tim-1 on T cells.

NCBI and Uniprot Product Information

• NCBI GI #: 110624776
• NCBI Accession #: NP_848874.3
• NCBI GenBank Nucleotide #: NP_848874.3
• UniProt Accession #: Q6U7R4
• NCBI Official Full Name: T-cell immunoglobulin and mucin domain-containing protein 4
• UniProt Protein Name: T-cell immunoglobulin and mucin domain-containing protein 4
• UniProt Gene Name: Timd4
• UniProt Synonym Gene Names: Tim4; TIMD-4; SMUCKLER; TIM-4
• UniProt Entry Name: TIMD4_MOUSE

Uniprot Description

TIM-4: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1. Belongs to the immunoglobulin superfamily. TIM family.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane