Western Blot (WB)
(The cell lysatederived from EGF-stimulated A431was resolved onto 12% SDS-PAGE and immunoblotted by Mouse anti pSER (MBS460004) at 1:500 (lane A); or pre-incubated by Phosphoserine (land B) or pre-incubated by phosphotyrosine (lnae C). A panel of phosphorylated proteins was observed)
Mouse Phosphoserine (Biotinylated) Antibody
Mouse anti Phosphoserine (pSER) antibody, Biotin Conjugated
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
The Mouse IgG1 is purified by affinity chromatography.
Synonyms
Phosphoserine (Biotinylated); Mouse anti Phosphoserine (pSER) antibody; Biotin Conjugated; Mouse anti-pSER; anti-Phosphoserine (Biotinylated) antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgG1, K
Clone Number
2A4
Specificity
This antibody recognizes phospho-serine (phospho-Ser) only. It does not cross react with phospho-Tyrosine or phospho-Threonine.
Purity/Purification
The Mouse IgG1 is purified by affinity chromatography.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-Phosphoserine (Biotinylated) antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Antigen Preparation
A chemically linked phosphoserine.
Positive Control
A431 (EGF stimulated)
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C.
The antibodies can be stored at 2°C to 8°C for one month without detectable loss of activity.
Avoid repeated freezing-thawing cycles.
The antibodies can be stored at 2°C to 8°C for one month without detectable loss of activity.
Avoid repeated freezing-thawing cycles.
Western Blot (WB)
(The cell lysatederived from EGF-stimulated A431was resolved onto 12% SDS-PAGE and immunoblotted by Mouse anti pSER (MBS460004) at 1:500 (lane A); or pre-incubated by Phosphoserine (land B) or pre-incubated by phosphotyrosine (lnae C). A panel of phosphorylated proteins was observed)
Western Blot (WB)
(The cell lysatederived from EGF-stimulated A431was resolved onto 12% SDS-PAGE and immunoblotted by Mouse anti pSER (MBS460004) at 1:500 (lane A); or pre-incubated by Phosphoserine (land B) or pre-incubated by phosphotyrosine (lnae C). A panel of phosphorylated proteins was observed)
Related Product Information for anti-Phosphoserine (Biotinylated) antibody
Background: Protein phosphorylation is involved in cell signaling pathways. These cascades are mediated by two types of kinases: serine/threonine kinases which phosphorylate serine and threonine amino acid side chains and tyrosine kinases which phosphorylate tyrosine amino acid side chains. This process is regulated by kinases and phosphatases.
Similar Products
Product Notes
The Phosphoserine (Biotinylated) (Catalog #AAA460005) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Phosphoserine (pSER) antibody, Biotin Conjugated reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoserine (Biotinylated) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the Phosphoserine (Biotinylated) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphoserine (Biotinylated), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
