Testing Data
Rabbit MEK1/2 (paired S218/S222) Antibody | anti-MEK1/2 (paired S218/S222) antibody
Rabbit anti MEK1/2 (Non- Phosphospecific)
Reactivity
Human, Mouse, Rat, Chicken
Applications
ELISA, Western Blot
Purity
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Synonyms
MEK1/2 (paired S218/S222); Rabbit anti MEK1/2 (Non- Phosphospecific); Rabbit anti-MEK1/2 (Nonphosphospecific); MAPKK1/2; MEK1/2.; anti-MEK1/2 (paired S218/S222) antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken
Specificity
This antibody recognizes ~44/45 kDa of human MEK1 protein without phosphorylation sites of Ser 218/222 or MEK2 (pS222/226). It does not cross react to phospho MEK1/2. This antibody also reacts with mouse and rat, chicken. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-MEK1/2 (paired S218/S222) antibody
ELISA, Western Blot
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Antigen Preparation
A synthetic peptide surrounding the epitope -SMANS- without phosphorylation of human MEK1/2. This sequence is identical among human, rat, mouse, chicken.
Positive Control
3T3
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-MEK1/2 (paired S218/S222) antibody
The MEK1 & 2 (MAPKK1/2) are members of tyrosine/threonine protein kinase family that activate the ERK1+2/MAPK enzymes by phosphorylation. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEKs. The MEK1 & 2 are regulated by phosphorylation by one of the MEK kinases.
References
Ali R. Jazirehi, et al. Inhibition of the Raf-MEK1/2-ERK1/2 Signaling Pathway, Bcl-xL Down-Regulation, and Chemosensitization of Non-Hodgkin’s Lymphoma B Cells by Rituximab. CANCER RESEARCH 64, 7117-7126, October 1, 2004
Similar Products
Product Notes
The MEK1/2 (paired S218/S222) (Catalog #AAA462073) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti MEK1/2 (Non- Phosphospecific) reacts with Human, Mouse, Rat, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's MEK1/2 (paired S218/S222) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the MEK1/2 (paired S218/S222) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK1/2 (paired S218/S222), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
