Western Blot (WB)
(Western blot analysis of A431 cells treated with calyculin A (100nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.)
Rabbit Phosphoserine/threonine Polyclonal Antibody
Phosphoserine/threonine Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry
Synonyms
Phosphoserine/threonine; Polyclonal Antibody; Phosphoserine/threonine Antibody; Anti-Phosphoserine/threonine; Phosphoser/thr pAb; anti-Phosphoserine/threonine antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to unphosphorylated peptide then affinity purified using a mix of phosphoserine and phosphothreonine peptides (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Phosphoserine/threonine antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
ELISA: 1:2000
ICC: 1:50
IP: 1:100
WB: 1:1000
ICC: 1:50
IP: 1:100
WB: 1:1000
Immunogen
Anti-Phosphoserine/threonine was generated from a panel of phosphoserine and phosphothreonine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved in many species.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of A431 cells treated with calyculin A (100nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.)
Western Blot (WB)
(Western blot analysis of A431 cells treated with calyculin A (100nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.)
Testing Data
(Bar grapHshowing anti-Phosphoserine/threonine (PP2551) binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.)
Testing Data
(Bar grapHshowing anti-Phosphoserine/threonine (PP2551) binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.)
Related Product Information for anti-Phosphoserine/threonine antibody
Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.
References
Yaffe, M.B. & Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
Krishna, R.G. & Wold, F. (1993) Adv Enzymol Rel Areas Mol Biol 67:265.
Hunter T.(1987) Cell. 50(6):823.
Krishna, R.G. & Wold, F. (1993) Adv Enzymol Rel Areas Mol Biol 67:265.
Hunter T.(1987) Cell. 50(6):823.
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Product Notes
The Phosphoserine/threonine (Catalog #AAA474532) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phosphoserine/threonine Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoserine/threonine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:50 IP: 1:100 WB: 1:1000. Researchers should empirically determine the suitability of the Phosphoserine/threonine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphoserine/threonine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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